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提高转基因效率，建立高效自杀基因杀伤胃癌肿瘤细胞的方法。方法：首先构建含CD基因的重组腺病毒载体并对载体进行包装、收获病毒上清并纯化。将重组病毒液转染MFC胃癌细胞进行体外抑瘤实验。结果：含CD基因的重组腺病毒载体经酶切鉴定选出：包装纯化后，检测病毒浓度为1.2×1012颗粒/ml。体外实验中，转CD基因的MFC细胞在5-FC作用下生长明显受抑(P<0.01,n＝9)。结论：腺病毒载体转导的CD基因提高了转染效率，为大量杀伤胃癌细胞奠定了基础。
Improve the efficiency of genetically modified, the establishment of efficient suicide gene killing of gastric cancer cells. Methods: Firstly, the recombinant adenovirus vector containing CD gene was constructed and the vector was packaged. The virus supernatant was harvested and purified. The recombinant virus was transfected into MFC gastric cancer cells in vitro anti-tumor experiments. Results: The recombinant adenovirus vector containing CD gene was selected by restriction enzyme digestion: After the package was purified, the virus concentration was detected as 1.2 × 1012 particles / ml. In vitro, MFC cells transfected with CD gene were significantly inhibited by 5-FC (P <0.01, n = 9). CONCLUSION: CD gene transduced by adenoviral vector enhances transfection efficiency and lays the foundation for large-scale killing of gastric cancer cells.