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背景与目的:研究证实垂体肿瘤转化基因1(pituitary tumor transforming gene 1,PTTG1)的表达情况与各类肿瘤细胞的恶性程度有关,但是其在胶质瘤中的作用尚不清楚。本研究旨在探讨PTTG1在恶性胶质瘤侵袭中的作用及其临床意义。方法:应用蛋白质印迹法(Western blot)检测PTTG1蛋白在不同胶质瘤细胞系中的表达;采用小RNA质粒干扰技术抑制U87细胞中PTTG1蛋白的表达,应用Western blot技术检测转染的效率;通过体外侵袭实验检测转染后细胞侵袭力的变化;采用Western blot技术检测经过表皮细胞生长因子(epithelial growth factor,EGF)刺激后敲除PTTG1的细胞组(siPTTG1/U87)与转染空载的细胞组(Scr/U87)中Akt、ARK5磷酸化的情况。结果:PTTG1蛋白在各恶性胶质瘤细胞系中均高表达;敲除PTTG1后U87细胞的侵袭力明显下降;对Scr/U87细胞进行EGF刺激5 min后,Akt、ARK5的磷酸化情况显著增强,而无论有无EGF的刺激,siPTTG1/U87中Akt、ARK5的磷酸化情况都没有明显改变。结论:在恶性胶质瘤细胞中,PTTG1蛋白呈高表达状态并且与侵袭性显著相关,其对侵袭性的调控可能是通过Akt-ARK5通路实现的。
BACKGROUND & AIM: The study confirmed that the expression of pituitary tumor transforming gene 1 (PTTG1) is related to the malignant degree of various tumor cells. However, the role of PTTG1 in gliomas remains unclear. This study aimed to investigate the role of PTTG1 in the invasion of malignant glioma and its clinical significance. METHODS: The expression of PTTG1 protein in different glioma cell lines was detected by Western blot. The expression of PTTG1 protein in U87 cells was inhibited by small RNA interference. The transfection efficiency was detected by Western blot. The invasiveness of cells transfected with PTTG1 (siPTTG1 / U87) stimulated by EGF was detected by Western blot and the cells transfected with empty vector Akt, ARK5 phosphorylation in the group (Scr / U87). Results: PTTG1 protein was highly expressed in all malignant glioma cell lines. The invasiveness of U87 cells was significantly decreased after knockdown of PTTG1. The phosphorylation of Akt and ARK5 was significantly increased in Scr / U87 cells stimulated with EGF for 5 min , And the phosphorylation of Akt and ARK5 in siPTTG1 / U87 did not change significantly with or without EGF stimulation. Conclusions: In malignant glioma cells, PTTG1 protein is highly expressed and is significantly associated with invasiveness. Its regulation of invasion may be through the Akt-ARK5 pathway.