目的:研究海带多糖在大鼠动脉粥样硬化中的预防作用。方法:采用高脂饲料喂饲+VitD3复制大鼠粥样动脉硬化模型。SD大鼠随机分为六组:正常组、模型组、阳性药组、海带多糖1000mg/kg组、海带多糖500mg/kg组和海带多糖250mg/kg组。造模给药12周,颈动脉取血,检测血清TG、TC、HDL-C和LDL-C水平。蛋白免疫印迹、逆转录聚合酶反应法检测细胞间粘附分子(ICAM-1)、血管粘附分子(VCAM-1)的蛋白与mRNA表达。结果:海带多糖高剂量组(1000mg/kg)能够显著降低血清TC、TG和LDL-C,提高HDL-C的水平,且明显抑制主动脉ICAM-1、VCAM-1的表达。结论:海带多糖对高脂饲料诱导的大鼠动脉粥样硬化具有保护作用,其机制可能与其调节血脂,调节细胞粘附因子分泌有关。 Objective: To study the preventive effect of kelp polysaccharide on atherosclerosis in rats. Methods: Rat model of atherosclerosis was induced by high fat diet and VitD3. SD rats were randomly divided into six groups: normal group, model group, positive drug group, kelp polysaccharide 1000mg / kg group, kelp polysaccharide 500mg / kg group and kelp polysaccharide 250mg / kg group. After 12 weeks of administration, blood samples were taken from the carotid artery to detect serum TG, TC, HDL-C and LDL-C levels. Western blotting and RT-PCR were used to detect the protein and mRNA expression of intercellular adhesion molecule (ICAM-1) and vascular adhesion molecule (VCAM-1). Results: The high dose of kelp polysaccharide (1000mg / kg) significantly decreased serum TC, TG and LDL-C, increased the level of HDL-C and significantly inhibited the expression of ICAM-1 and VCAM-1 in the aorta. Conclusion: Laminaria polysaccharide has a protective effect on atherosclerosis induced by high-fat diet in rats. The mechanism may be related to the regulation of blood lipids and the secretion of adhesion molecules.