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目的明确离子液体作流动相添加剂高效液相法分离生物碱的过程和作用机理。方法利用离子液体作流动相添加剂,高效液相法分离苦参类生物碱,并根据2个经典的保留模型:溶质计量置换保留模型(SDM-R)和Snyder经验式,建立模型,考察离子液体浓度,烷基链长度和有机溶剂体积比对组分保留行为的影响,通过模型参数研究保留过程和保留机理。结果当离子液体作流动相添加剂,并达到一定浓度时,离子液体的浓度与组分的保留值符合SDM-R模型的线性关系,且Z值随离子液体烷基链长度的增加而减小,logI值增大。结论离子液体作为流动相添加剂时,生物碱的分离保留过程以竞争吸附作用为主,且符合SDM-R模型。
Objective To clarify the process and mechanism of high performance liquid phase separation of alkaloids using ionic liquids as mobile phase additives. Methods Ionic liquid as mobile phase additive was used to separate the alkaloids from Sophora flavescens. HPLC-MS method was used to establish the model according to two classical retention models: SDM-R and Snyder empirical formula. Concentration, alkyl chain length and volume ratio of organic solvent on the retention behavior of the components were studied. The retention process and mechanism of retention were studied by model parameters. Results When the ionic liquid was used as the mobile phase additive and reached a certain concentration, the concentration of the ionic liquid and the retention of the components were in accordance with the linear relationship of the SDM-R model. The Z value decreased with the increase of the alkyl chain length of the ionic liquid, logI value increases. Conclusion When ionic liquid is used as mobile phase additive, the separation and retention process of alkaloids is dominated by competitive adsorption and accords with SDM-R model.