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[目的]研究含氟茶浸泡液的细胞毒性和致突变性,探讨高氟茶对中国仓鼠肺成纤维细胞(V79细胞)的遗传毒作用。[方法]选取绿茶A、绿茶B、对照茶为研究对象,分别制备其茶浸泡液。将上述3种茶浸泡液分别设立0.63、1.25、2.50、5.00、10.00、20.00、40.00g/L7个质量浓度组,对V79细胞分别进行2、4、8、12、24h染毒。采用甲基四唑蓝(MTT)法检测3种茶浸泡液对V79细胞的毒性;运用鼠伤寒沙门菌回复突变试验(Amestest)检测3种茶浸泡液的致突变性。[结果]绿茶A、绿茶B和对照茶浸泡液中氟化物的浓度分别为2330、1390、7.53mg/kg,绿茶A、绿茶B的氟含量均超过了中国农业行业标准(NY659—2003)规定茶叶中氟化物(以F-计)≤200mg/kg的标准。在MTT实验中,3种茶浸泡液对细胞活性的抑制作用具有明显的时间-效应(P<0.05)和剂量-效应关系(P<0.05)。绿茶A、绿茶B的细胞存活率明显低于对照茶(P<0.05)。在作用4￣24h时,绿茶A的细胞存活率低于绿茶B(P<0.05)。分析3种茶不同培养时间的半数抑制浓度(IC50)发现,3种茶组间IC50的差异均有统计学意义(P<0.05),绿茶A、绿茶B、对照茶不同培养时间平均IC50分别为20.26、23.24、28.18g/L。在Ames试验中,3种茶浸泡液在8￣5000μg/皿的浓度上,4菌株回变菌落数与相应的阴性对照组之间的差异均有统计学意义。[结论]绿茶A、B浸泡液对V79细胞活性均有抑制作用,其细胞毒性均明显高于对照茶。未见高氟茶浸泡液有致突变作用。绿茶A、B对V79细胞的毒性比对照组大可能与它含氟量高有关。
[Objective] To investigate the cytotoxicity and mutagenicity of fluoride-containing tea infusion solution and to explore the genetic toxic effects of high-fluoride tea on Chinese hamster lung fibroblasts (V79 cells). [Method] Green tea A, green tea B and control tea were selected as the research object, and their tea infusion solutions were prepared. The above three kinds of tea soaking solution were set at 0.63, 1.25, 2.50, 5.00, 10.00, 20.00 and 40.00 g / L, respectively. The V79 cells were exposed to 2,4,8,12,24h respectively. The toxicity of three kinds of tea soaking solution to V79 cells was detected by methyltetrazolium blue (MTT) method. The mutagenicity of three kinds of tea soaking solution was tested by salmonella typhimurium back mutation test (Amestest). [Result] The fluoride concentrations in green tea A, green tea B and control tea soaking solution were 2330, 1390 and 7.53 mg / kg, respectively. The contents of fluorine in green tea A and green tea B exceeded the requirements of China’s agricultural industry standard (NY659-2003) Fluoride in tea (F-meter) ≤ 200mg / kg standard. In the MTT experiment, three kinds of tea infusion had obvious time-effect (P <0.05) and dose-effect relationship (P <0.05) on the inhibition of cell viability. Green tea A, green tea B cell survival was significantly lower than the control tea (P <0.05). The cell viability of green tea A was lower than that of green tea B at 4 ~ 24h (P <0.05). The IC50 values of three kinds of tea at different culture time were analyzed. The differences of IC50 among three kinds of tea were statistically significant (P <0.05). The average IC50 of different culture time of green tea A, green tea B and control tea were 20.26, 23.24, 28.18 g / L. In the Ames test, the difference between the number of colonies returned by 4 strains and the corresponding negative control group was statistically significant at the concentrations of 8 to 5000 μg / dish in the three tea infusion solutions. [Conclusion] The green tea A and B soaking liquid all inhibited the activity of V79 cells, and their cytotoxicity was significantly higher than that of the control tea. No high-fluorine tea soaked liquid mutagenic effect. Green tea A, B of V79 cells toxicity than the control group may be related to its high fluoride content.