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作者对7例确诊的缺铁性贫血病人和7例骨髓移植供者作了一系列的幼红细胞超微结构研究。方法:常规法从病人和对照组(骨髓移植供者)胸骨穿取骨髓,直接放入预冷含2%戍二醛的0.1M磷酸盐缓冲液(PBS)中,分离出髓粒与新鲜的2%戍二醛混合置4℃2小时,用0.1MPBS蔗糖液轻轻洗涤,混合后置四氧化锇中,丙酮梯度洗脱,环氧树脂包埋,用Reichert’s超薄切片机切片,置铜网上,用巯基醋酸铀和枸橼酸铅染色,Jel 100B透射电镜
The authors made a series of studies on erythrocyte ultrastructure in seven patients with iron-deficiency anemia and seven patients with bone marrow transplantation. METHODS: Bone marrow was routinely perfused from the sternum of patients and controls (bone marrow donor) directly into 0.1 M phosphate buffered saline (PBS) containing 2% glutaraldehyde, and isolated from the medulla with fresh 2% glutathione mixed set 4 ℃ 2 hours, gently washed with 0.1MPBS sucrose solution, mixed with osmium tetroxide, acetone gradient elution, embedded in epoxy resin, sliced with Reichert’s ultra-thin slicer, set the copper Online, stained with mercaptoacetic acid uranium and lead citrate, Jel 100B transmission electron microscopy