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目的:观察利用siRNA技术下调人脑胶质母细胞瘤细胞(U251)STAT3表达后对细胞凋亡的影响。方法:分离培养并间接免疫荧光鉴定大鼠原代星形胶质细胞(NA),用STAT3干涉质粒载体(pSilence2.1-STAT3)及对照载体(pSilence2.1-GFP)转染U251细胞及NA细胞,用Hoechst 33258染色观察凋亡的形态学改变,用Annexin-V试剂盒定量检测凋亡。结果:分离培养了符合实验要求的NA细胞,转染pSilence2.1-STAT3后,U251细胞出现了明显的时间依赖性细胞凋亡,而NA细胞无显著凋亡发生。结论:pSilence2.1-STAT3能诱导U251细胞凋亡,而对正常细胞无显著影响。
OBJECTIVE: To observe the effects of siRNA on the apoptosis of human glioblastoma cells (U251) induced by STAT3. Methods: Primary rat astrocytes (NA) were isolated and cultured with indirect immunofluorescence. U251 cells and NA were transfected with pSilence2.1-STAT3 and pSilence2.1-GFP Cells were stained with Hoechst 33258 to observe the morphological changes of apoptosis, and apoptosis was quantitatively detected by Annexin-V kit. Results: NA cells were isolated and cultured. After transfection with pSilence2.1-STAT3, U251 cells showed significant time-dependent apoptosis, while NA cells showed no significant apoptosis. Conclusion: pSilence2.1-STAT3 can induce apoptosis of U251 cells, but has no significant effect on normal cells.