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通过层层自组装的方法,在中性条件下利用静电作用将Fe3O4纳米粒子组装到修饰了高分子聚电解质的CaCO3多孔微米球上。该复合材料具有好的生物相容性、导电性、磁性和稳定性。将血红蛋白酶固定到该复合材料上,进而制备得到血红蛋白(Hb)-Fe3O4-CaO3复合物修饰玻碳电极,并在该修饰电极上实现了Hb与电极之间的直接电化学。该生物传感器对H2O2的还原具有较好的响应,线性范围为3.0×10-6~5.3×10-5 mol/L,检测限为8.9×10-7 mol/L。
Through the method of layer self-assembly, the Fe3O4 nanoparticles were assembled into CaCO3 porous microspheres modified with polymer polyelectrolytes under neutral conditions. The composite has good biocompatibility, conductivity, magnetism and stability. Hemoglobin (Hb) -Fe3O4-CaO3 complex modified glassy carbon electrode was prepared by immobilizing hemoglobin on the composite material, and direct electrochemical between Hb and electrode was achieved on the modified electrode. The biosensor has a good response to the reduction of H2O2 with a linear range of 3.0 × 10-6 ~ 5.3 × 10-5 mol / L and a detection limit of 8.9 × 10-7 mol / L.