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应用RT PCR和DNA克隆重组技术 ,从中国云南地区丙型肝炎患者血清中克隆到 3个丙型肝炎病毒 (HCV) 5′端非编码区 (5′NCR)核苷酸序列。这 3个序列分别来自 3个不同的丙型肝炎患者。与国内外已发表的HCV原型株同源序列比较研究发现 ,这 3个HCV 5′NCR序列中分别存在部分核苷酸序列的缺失或出现插入重复。在YS2 2 5 15′NCR序列 - 15 5～ - 174位之间 ,有 18个核苷酸序列缺失 ;在YS2 0 3 4和YS2 42 15′NCR序列 - 5 5～ - 5 6位之间 ,分别有 2 8个 (YS2 0 3 4)和 40个 (YS2 42 1)核苷酸序列插入。这些插入序列 (2 8个核苷酸 )在其相邻部位已有存在 ,即为重复序列。YS2 42 1插入序列中有 11个核苷酸出现两次重复。其它部位的核苷酸变异也同已知基因型的相应核苷酸变异有很大差异。结果表明 ,这 3个序列是迄今为止国内外都未曾发现过的新的HCV核苷酸序列
Three 5 ’untranslated region (5’NCR) nucleotide sequences of hepatitis C virus (HCV) were cloned from the serum of patients with hepatitis C in Yunnan, China by RT PCR and DNA cloning recombination. These three sequences were from three different hepatitis C patients. Compared with published homologous sequences of HCV prototype strains both at home and abroad, it was found that some nucleotide sequences were deleted or inserted repeatedly in the 3 5’NCR sequences. Between YS2 2 5 15’NCR sequence -15 5 ~ -174, 18 nucleotides were deleted; between YS2 0 3 4 and YS2 42 15’NCR sequence-5 5 ~-5 6, There are 2 8 (YS2 0 3 4) and 40 (YS2 42 1) nucleotide sequences inserted respectively. These insertions (28 nucleotides) are already present in their neighbors, ie as repeats. There are 11 repeats in the YS2 42 1 insertion sequence with two repeats. The nucleotide variation at other sites is also quite different from the corresponding nucleotide variation at known genotypes. The results showed that these three sequences were new HCV nucleotide sequences