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目的:探索慢性髓性白血病（CML）患者外周血样本的存储和运输对实时定量PCR（RQ-PCR）检测BCR-ABL（P210）转录本水平的影响。方法:采集84例CML患者外周血样本，根据储存时间（0、6、12、24、48和72 h）、温度[室温（18~24 ℃）和低温（2~8 ℃）]以及振荡条件（振荡时长3、6和12 h）设置不同分组，RQ-PCR法检测不同条件下外周血样本BCR-ABL（P210）转录本水平。本研究将BCR-ABL拷贝数、ABL拷贝数和BCR-ABL（P210）转录本水平等原始数据进行对数转化（logn 10N）。n 结果:①琼脂糖凝胶电泳结果显示：室温条件下，储存48、72 h的样本RNA出现显著降解；②储存温度方面，总体样本中，室温储存48、72 h的样本BCR-ABL拷贝数检测水平显著低于低温储存的样本（n P0.05）；③储存时间方面，与基线的3.35±1.60相比，总体样本的BCR-ABL拷贝数检测结果仅在室温储存储存下48 h（2.93±1.59）和72 h（2.79±1.42）显著下降（n P0.05）。n 结论:样本储存时间、储存温度及运输振荡因素可以影响BCR-ABL、ABL拷贝数的检测结果，但对BCR-ABL（P210）转录本水平定量检测结果无显著影响。“,”Objective:To explore the influence of storage and delivery conditions of the peripheral blood samples from patients with chronic myeloid leukemia (CML) on the real-time quantitative PCR (RQ-PCR) detection of the BCR-ABL (P210) transcript levels.Methods:The peripheral blood samples of 84 CML patients were collected. The same sample was divided into different groups according to storage time (0, 6, 12, 24, 48, and 72 h) , temperature (room temperature, 18-24 ℃; low temperature, 2-8 ℃) , and vibration conditions (3, 6, and 12 h) . RQ-PCR was used to detect BCR-ABL (P210) transcript levels of the different groups. This study logarithmically transformed (logn 10N) the original data [BCR-ABL copy number, ABL copy number, and BCR-ABL (P210) transcript levels].n Results:①Agarose gel electrophoresis showed significant RNA degradation of samples after storage for 48 and 72 h at room temperature. ②Among the overall samples, the BCR-ABL copy number of the samples stored at room temperature for 48 and 72 h was significantly lower than that of the samples stored at low temperature (n P0.05) compared with that at baseline (0 h, -0.56±1.51) . ④ The BCR-ABL copy number of the overall sample only decreased significantly (n P<0.05) at 48 h (2.93±1.59) and 72 h (2.79±1.42) compared with that at baseline (0 h, 3.35±1.60) when stored at room temperature. The ABL copy number in the overall sample decreased significantly at 48 and 72 h (whether low and room temperature;n P0.05) compared with that at baseline (0 h, -0.60±1.37) .n Conclusion:Sample storage time, storage temperature, and vibration can interfere with the results of BCR-ABL and ABL copy number but have no significant effect on the quantitative determination of BCR-ABL (P210) transcript levels. This study provides strong support for the feasibility of transregional transportation of peripheral blood samples from patients with CML.