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目的观察黄精对自然衰老大鼠骨髓内皮祖细胞(endothelial progenitor cells,EPCs)功能及端粒酶活性的影响。方法 48只自然衰老大鼠(20月龄),按体重随机分为空白组,黄精高、中、低剂量组,每组12只;同时取12只青年大鼠(5月龄)作为正常对照组。黄精高、中、低剂量组大鼠分别予以4、2、1g/kg黄精水煎液灌胃,同时正常对照组和空白组予以等量生理盐水灌胃,每日1次,连续12周。检测大鼠抗氧化指标的含量,同时分离培养各组大鼠骨髓单个核细胞后对贴壁细胞进行鉴定;采用四唑盐(MTT)法检测各组细胞增殖力、迁移小室检测迁移力,体外血管生成试剂盒检测成小管功能;实时荧光定量PCR法检测端粒酶活性。结果与正常对照组比较,空白组血清总抗氧化力(total-antioxidant capacity,T-AOC)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽-过氧化物酶(glutathione-peroxidase,GSH-Px)含量明显减少(P<0.01),丙二醛(malondialdehyde,MDA)含量增加(P<0.01),骨髓EPCs功能受损明显,细胞端粒酶活性降低(P<0.01);与空白组比较,黄精高、中剂量组血清T-AOC、SOD和GSH-Px含量增加(P<0.05,P<0.01),MDA含量减少(P<0.05,P<0.01),黄精高、中剂量组均可促进EPCs的增殖、迁移、成小管功能和提高细胞端粒酶活性(P<0.05,P<0.01)。结论黄精可通过促进血清抗氧化水平和细胞端粒酶活性来保护衰老大鼠受损的EPCs功能。
Objective To observe the effect of Polygonatum sibiricum on the function and telomerase activity of bone marrow endothelial progenitor cells (EPCs) in rats with natural aging. Methods Forty-eight aged rats (20 months old) were randomly divided into blank control group, high-dose group and low-dose group with 12 rats in each group. Twelve young rats (5 months old) group. Huangjing high, medium and low dose groups were given 4,2,1 g / kg Polygonatum decoction gavage, while normal control group and blank group were given normal saline, once a day for 12 weeks. The content of anti-oxidative index in rat was detected, and the adherent cells were identified after isolation and culture of rat bone marrow mononuclear cells. The proliferation of cells in each group was detected by MTT assay. The angiogenesis kit was used to detect the function of small tubules; telomerase activity was detected by real-time fluorescence quantitative PCR. Results Compared with the normal control group, the levels of total-antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione-peroxidase (P <0.01). The contents of malondialdehyde (MDA) were increased (P <0.01), the function of bone marrow EPCs was significantly impaired, and the telomerase activity was decreased (P <0.01) The contents of T-AOC, SOD and GSH-Px in the Huangjing high and middle dose groups were significantly increased (P <0.05, P <0.01) All of them can promote EPCs proliferation, migration, tubule function and cell telomerase activity (P <0.05, P <0.01). Conclusion Polygonatum can protect the damaged EPCs function in aging rats by promoting serum anti-oxidant level and telomerase activity.