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研究了基质金属蛋白酶基因MMP20在人食管癌发生发展过程中的表达变化. 用RT-PCR检测配对食管癌手术标本及食管癌早期活检组织中MMP20基因表达, 用免疫组织化学染色和Western印迹检测表达的MMP20蛋白. 发现MMP20基因在mRNA和蛋白质水平上均为食管癌过表达, MMP20基因的表达在食管癌发生早期未见明显升高, 在浸润癌期明显过高表达, 提示在食管癌发展中MMP20过表达与癌细胞浸润相关, 是食管癌演进过程中的晚期事件.
The expression of matrix metalloproteinase gene MMP20 in the development of human esophageal cancer was studied. RT-PCR was used to detect the expression of MMP20 gene in paired esophageal cancer surgical specimens and biopsy tissues of esophageal cancer. The expression was detected by immunohistochemical staining and Western blot. MMP20 protein. MMP20 gene was found to be overexpressed in both mRNA and protein levels. The expression of MMP20 gene was not significantly elevated in the early stage of esophageal cancer, and it was significantly over expressed in invasive stage, suggesting that esophageal cancer is developing. MMP20 overexpression is associated with cancer infiltration and is a late event in the evolution of esophageal cancer.