取花蕾长3.0~5.5mm的花序,7%(W/V)饱和次氯酸钙溶液(加一滴吐温20)表面消毒15min,无菌水洗3次.然后轻压花蕾,过滤,收集滤液,离心后,将小孢子悬浮于经过滤灭菌的改良的1/2NLN培养基中.石蜡膜封口,置温度梯度培养箱作32.5℃、1d的高温热激诱导处理,后置25℃下继续培养.本试验对13份绿菜花供试材料的游离小孢子培养能力进行初步研究,其中8种基因型(占全部材料的61.5%)有胚胎发生,并获得5种基因型的再生株,共207株.基因型对绿菜花游离小孢子培养的影响很大,基因型“巴绿青花菜”出胚率最高,达37.08胚/蕾.同时,对培养基中加入活性炭悬浮液和更换培养基的作用进行了研究.“,”Buds of 3 0~5.5mm in length of broccoli (Brassica oleracea var.italica) were removed from the raceme,surface sterilized with full strength commercial bleach (7%sodium hypochlorite)for 15 min and rinsed 3 times with sterile deionized water.Then they were squeezed,filtered,prepared and centrifuged.The density of the suspension was cultured in modified liquid medium 1/2 NLN lacking growth substances.They were sealed with parafilm and incubated in dardness at 32.5℃ for one day and then incubated at 25℃ conti...