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目的:观察蝎毒多肽提取物(PESV)对Lewis肺癌(LLC)免疫逃逸的影响。方法:取40只C57BL/6J小鼠,右腋下接种Lewis肺癌细胞悬液,建立皮下种植瘤模型,随机分为荷瘤对照组和PESV治疗组,连续灌胃给予PESV 18 d,检测肿瘤体积并计算抑瘤率;免疫组化法和ELISA法分别检测瘤组织及血清中血管内皮生长因子(VEGF)、转化生长因子β1(TGF-β1)和白介素10(IL-10)的表达,免疫组化法和流式细胞仪分别检测肺癌组织中浸润的DC表面共刺激分子CD80,CD86的表达。结果:PESV抑瘤率为56.60%。与荷瘤对照组相比,瘤组织和血清中VEGF,TGF-β1和IL-10的表达明显降低(P<0.05),DC共刺激分子CD80,CD86的表达明显增加(P<0.05)。结论:PESV可干预肺癌免疫逃逸,其机制可能与减少肿瘤微环境中VEGF,TGF-β1和IL-10的表达,增加DC共刺激分子CD80,CD86的表达有关。
OBJECTIVE: To investigate the effect of scorpion venom polypeptide extract (PESV) on immune escape of Lewis lung cancer (LLC). METHODS: Forty C57BL/6J mice were selected and inoculated with Lewis lung cancer cell suspension in the right axilla to establish a subcutaneous implantable tumor model. They were randomly divided into a tumor-bearing control group and a PESV-treated group. PESV was continuously administered by gavage for 18 days to detect tumor volume. The tumor inhibition rate was calculated. The expression of vascular endothelial growth factor (VEGF), transforming growth factor β1 (TGF-β1), and interleukin 10 (IL-10) in tumor tissue and serum were detected by immunohistochemistry and ELISA, respectively. The expression of CD80 and CD86 on infiltrating DC surface co-stimulatory molecules in lung cancer tissues was detected by chemical method and flow cytometry. Results: The inhibitory rate of PESV was 56.60%. Compared with the tumor-bearing control group, the expression of VEGF, TGF-β1 and IL-10 in the tumor tissue and serum was significantly lower (P<0.05), and the expression of DC co-stimulatory molecules CD80 and CD86 was significantly increased (P<0.05). Conclusion: PESV can interfere with the immune escape of lung cancer. The mechanism may be related to the decrease of the expression of VEGF, TGF-β1 and IL-10 in the tumor microenvironment, and increase of the expression of DC co-stimulatory molecules CD80 and CD86.