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With phosphotriester approach, the 2’—5’ linked oligonucleotide——2’—5’A core was synthesized by using cross-linked polyacrylmorpholide resin as the support and the mesitylene sulfonyl chloride and N-methylimidazole as the condensing agent. Synthesizing the 2’—5’A core with this method has not yet been described. The yields that each of the protected monomers was attached to the polymeric support were satisfactory, i.e., 100%, 60% and 91% respectively.For the synthesis, we improved the methods for preparing the polymeric support and a series of intermediates. For this reason, the yields of the products were markedly increased and the operations to separate the products from the by-products and reagents were simplified.
With phosphotriester approach, the 2’-5 ’linked oligonucleotide - 2’-5’A core was synthesized by using cross-linked polyacrylmorpholide resin as the support and the mesitylene sulfonyl chloride and N-methylimidazole as the condensing agent. Synthesizing the 2 ’-5’A core with this method has not yet been described. The output that each attached the polymeric support were satisfactory, ie, 100%, 60% and 91% respectively. For the synthesis, we improved the methods for preparing the polymeric support and a series of intermediates. For this reason, the yield of the products were markedly increased and the operations to separate the products from the by-products and reagents were simplified.