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目的：研究肝素和氢化可的松以及二者联合应用对人脐静脉内皮细胞（ECV304细胞）和成纤维细胞（WI－38细胞）的生长抑制作用和细胞凋亡的影响，并比较普通肝素与低分子肝素作用的差异，探讨肝素和氢化可的松联合应用抑制血管生成的可能性。方法：将细胞在含有药物的培养液中孵育一定时间，观察其形态，用MTT法测定细胞的增殖抑制率，电镜下观察细胞凋亡情况和ELISA法定量检测细胞凋亡程度。结果：肝素和氢化可的松单独均可抑制ECV304细胞增殖，作用呈剂量依赖性；肝素对WI－38细胞亦有增殖抑制作用。肝素和氢化可的松联合应用具有协同作用。低分子肝素抑制作用强于普通肝素。经电镜观察和ELISA法检测，肝素和（或）氢化可的松没有明显诱导细胞凋亡作用。结论：肝素可抑制血管内皮细胞增殖但不具有特异性，且和氢化可的松具有协同作用。以上作用不是通过诱导细胞凋亡实现的。
Objective: To study the effects of heparin and hydrocortisone and their combination on the growth inhibition and apoptosis of human umbilical vein endothelial cells (ECV304 cells) and fibroblasts (WI-38 cells), and to compare the effects of unfractionated heparin with Differences in the role of low molecular weight heparin, to explore the combination of heparin and hydrocortisone inhibit the possibility of angiogenesis. METHODS: The cells were incubated in culture medium containing drugs for a certain period of time. The morphology was observed. The cell proliferation inhibition rate was measured by MTT assay. The cell apoptosis was observed under electron microscope and the apoptosis was quantified by ELISA. RESULTS: Heparin and hydrocortisone alone could inhibit the proliferation of ECV304 cells in a dose-dependent manner. Heparin also inhibited the proliferation of WI-38 cells. The combined use of heparin and hydrocortisone has a synergistic effect. The inhibitory effect of low molecular weight heparin is stronger than that of unfractionated heparin. Electron microscopy and ELISA showed that heparin and/or hydrocortisone did not significantly induce apoptosis. Conclusion: Heparin can inhibit the proliferation of vascular endothelial cells but is not specific, and has a synergistic effect with hydrocortisone. The above effects are not achieved by inducing apoptosis.