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将长２～３ｃｍ的小麦幼穗剪成２～３ｍｍ长的小切段接种于添加２，４－Ｄ、ＮＡＡ的ＭＳ培养基上，当愈伤组织直径达５ｍｍ左右时，不加分割直接转移到分化培养基上培养，获得５４个试管变态小穗，其颖片和小花柄明显伸长，呈簇生状或分枝状，比正常的大２～８倍，花器发育完全。在无菌条件下授以黑麦花粉，产生１１粒杂交种子。经离体胚培养，获得８株试管幼苗。
The young 2 ~ 3cm long wheat ears were cut into small pieces of 2 ~ 3mm in length and inoculated on 2,4-D and NAA-supplemented MS medium. When the callus diameter was about 5mm, the cuttings were directly transferred On differentiation medium, 54 test tube metamorphosis spikelets were obtained. The glume and the small flower stalk were obviously elongated, showing cluster-like or branched shape, which was 2 to 8 times larger than normal, and the floral organs developed completely. Rye pollen was grafted under aseptic conditions to yield 11 hybrid seeds. After in vitro embryo culture, 8 test-tube seedlings were obtained.