流式细胞仪(ＦＣＭ)已成为当代分析细胞学领域检测细胞分子特征的主要工具之一,在医学、生物学和肿瘤学等学科中得到广泛应用〔１,２〕。ＦＣＭ所用的鞘液是维持仪器正常运转的主要消耗试剂,其主要作用是经荧光染色的单细胞悬液和鞘液同时进入流动室,在气体压力的作用下,形成包 Flow cytometry (FCM) has become one of the most important tools in the contemporary analysis of molecular characteristics of cytology in the field of medicine, biology and oncology (1,2). The sheath fluid used for FCM is the main consumption reagent to maintain the normal operation of the instrument. The main function of the FCM is to enter the flow chamber simultaneously with the fluorescent single cell suspension and the sheath fluid under the action of gas pressure to form a bag