论文部分内容阅读
目的对未成熟卵的体外成熟(IVM)技术在体外受精-胚胎移植(IVF-ET)中的补救性应用进行总结,探讨影响其治疗结果的因素。方法在IVF-ET治疗中,对卵巢反应不良、卵泡发育迟缓和卵泡过多生长有发生过度刺激可能需要取消治疗周期的患者,在B超引导下经阴道卵泡穿刺术取卵后,对未成熟卵进行体外培养成熟后进行IVF或卵胞浆内单精子显微注射,良好胚胎进行移植。结果取卵周期33个,共获未成熟卵214个,进行体外培养后126个成熟;32个周期进行常规胚胎移植,8例临床妊娠,移植周期临床妊娠率为25.00%(8/32);7例分娩8个健康婴儿。结论未成熟卵IVM技术是IVF-ET治疗不孕不育中有效的补充方法,可以补救未成熟的卵,减少患者取消IVF-ET治疗周期的发生。
Objective To summarize the remedial use of in vitro fertilization-embryo transfer (IVF-ET) of immature ovum in vitro maturation (IVM) technique and to explore the factors influencing the outcome of treatment. Methods In the treatment of IVF-ET, ovarian dysfunction, follicular growth retardation and overexpression of follicles have over-stimulation may need to cancel the treatment cycle in patients undergoing transvaginal follicular puncture under B-guided ovulation, immature Eggs were cultured in vitro after IVF or intracytoplasmic sperm injection, good embryo transplantation. Results Totally 33 immature eggs were obtained, of which 214 were immature eggs. 126 embryos were matured in vitro. Conventional embryo transfer was performed in 32 cycles. The clinical pregnancy rate in 8 cycles was 25.00% (8/32). Seven patients gave birth to eight healthy infants. Conclusion Immature ovum IVM technique is an effective supplement for IVF-ET treatment of infertility, which can remedy immature ovaries and reduce the abolition of IVF-ET treatment cycle in patients.