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为明确成团泛菌Pantoea agglomerans引起的玉米细菌干茎腐病经种子传播的规律,采用细菌常规分离法、Sherlock微生物鉴定系统、特异性分子检测技术,对与干茎腐病相关的杂交种金玉9856及其父本PS056、母本OSL190进行了种子带菌检测,证明金玉9856和PS056种子内部带菌,获得分离物Pag1和Pag2,2个分离物对感病的PS056均具有致病性。菌液浸种、种子注射接种和自然带菌种子直播都能够引发干茎腐病,发病率分别达到100%、100%和80%,而持续高温(50℃)处理4天的种子则在植株上不表现症状。对种子接种后长成的植株的系统检测证明,成团泛菌侵染种子后,通过植株维管束系统向地上部组织扩展,随着水分的运输,病菌通过茎秆到达果穗的籽粒中,完成从种子到植株、再到新种子的病害循环,同时能够引起植株发病。
In order to clarify the rule of seed propagation of corn stalk rot stem rot caused by Pantoea agglomerans, bacterial routine separation method, Sherlock microbial identification system and specific molecular detection technique were used to analyze the stem-rot disease-related hybrid jade 9856 and its male parent PS056 and female parent OSL190. The results showed that Jinyu 9856 and PS056 were infected with Pag1 and Pag2 isolates, and both isolates were pathogenic to susceptible PS056. Seed soaking, seed inoculation, and live seed-borne seeding all can cause stem-stem rot with the incidence of 100%, 100% and 80% respectively, while the seeds treated with continuous high temperature (50 ℃) for 4 days are not on the plants Performance symptoms. Systematic examination of plants grown after seed inoculation demonstrated that after Pantoea agglomerans infects the seed, it expands to shoots through the vascular bundle system of the plant and, with the transport of moisture, the germs reach the ears of the grain through the stem, completing From seeds to plants, to the new seed disease cycle, at the same time can cause the plant disease.