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通过关联分析法发掘与花生(Arachis hypogaea)产量性状显著关联、同时又在花生基因组上随机分布的SSR位点及优异等位变异,可了解产量相关基因区域的分布特点,有助于利用分子标记辅助选择方法选育高产花生新品种。选用64个SSR标记,采用MLM(Q+K)方法对166份花生资源进行全基因组关联分析。结果表明,通过聚类分析和结构划分,供试群体受其综合性状遗传特点和来源地域的影响可被划分成7个亚群,聚类结果与群体结构基本一致,同时群体特点与材料来源地的生态划分符合同类聚集的规律。通过对6个产量相关性状的3年数值的关联分析,分别发掘出SSR位点有20个、33个和26个,2年以上重复检出的SSR位点有13个(P<0.05),各SSR位点的表型变异解释率范围为0.011–0.348 1,平均为0.067 3;共检出590个等位变异,平均每个标记位点12.29个,表型变异解释率值最高的是与单株果数呈显著关联的位点TC1A02(P<0.001),含21个等位变异;与产量构成主要因子紧密关联的位点中,百果重的TC1A02-C470(+41.588 5)、TC1A02-C560(+40.926 1)和p PGPseq2E6-B473(+63.953 4),单株果数的TC1A02-C500(+7.374 4),单株饱果数的GM1843-E157(+4.316 6),可用于产量性状的分子辅助育种。
Correlation analysis was used to discover the significant association of yield traits with Arachis hypogaea and the random distribution of SSR loci and excellent allelic variations in the peanut genome to understand the distribution characteristics of yield-related gene regions and facilitate the use of molecular markers Auxiliary selection method to breed new varieties of high yield peanut. Sixty-four SSR markers were used to analyze the genome-wide association of 166 peanut resources using MLM (Q + K) method. The results showed that the cluster could be divided into seven subgroups according to the genetic characteristics of the comprehensive traits and the geographical area of origin by cluster analysis and structure classification. The clustering results were basically consistent with the population structure, The ecological division accords with the law of the same kind of aggregation. According to the 3-year numerical analysis of 6 yield-related traits, 20, 33 and 26 SSR loci were found, respectively, and 13 SSR loci were detected repeatedly at 2 years (P <0.05) The explained rate of phenotypic variation for each SSR loci ranged from 0.011 to 0.348 1 with an average of 0.067 3. A total of 590 alleles were detected with an average of 12.29 loci per locus. The highest explained rate of phenotypic variation was Among the loci closely related to the main factors of yield formation, TC1A02-C470 (+41.588 5), TC1A02 (+40.926 1) and p PGPseq2E6-B473 (+63.953 4), TC1A02-C500 (+7.374 4) per plant and GM1843-E157 (+4.316 6) Molecular assisted breeding traits.