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目的评估一种条带免疫印迹试剂确证艾滋病病毒(HIV)抗体的效果。方法用蛋白印迹试验(WB)和条带免疫印迹试剂(LIA)平行检测1 035份标本,按照各自的说明书判断结果,对检测结果进行比较和分析。结果总体上,两种试剂检测的一致率为95.3%,对经过确证的640份HIV抗体阳性标本(含2份HIV-1O组和2份HIV-2标本)的检测的结果完全一致。在339份WB试剂判断为阴性的标本中,LIA试剂有5份(1.5%)为不确定结果,而在362份LIA试剂判断为阴性的标本中,WB试剂有28份(7.7%)为不确定结果(χ~2=15.29,P<0.001)。在初次检测的341份标本中,确证了17份为HIV抗体阳性,考核试剂检测5份为不确定,12份为阳性。结论 LIA试剂显示出非特异反应暨不确定结果少的优势,可考虑用于HIV抗体的常规确证,并在实际应用中不断改进完善。
Objective To assess the effectiveness of a banding immunoblot assay to confirm the presence of HIV antibodies. Methods 1 035 samples were detected in parallel by Western blotting (WB) and western blotting (LIA). The results were compared and analyzed according to the respective instructions. Results Overall, the agreement between the two reagents was 95.3%, and the results of the validated tests of 640 positive HIV antibody samples (including 2 HIV-1O and 2 HIV-2 samples) were completely consistent. Of the 339 samples that were negative for WB Reagent, 5 (1.5%) of the LIA Reagents were inconclusive, while of the 362 samples that were negative for the LIA Reagent, 28 (7.7%) of the WB Reagents were not Results were determined (χ ~ 2 = 15.29, P <0.001). Of the 341 samples initially tested, 17 were positive for HIV antibodies, 5 were undetected for testing reagents and 12 were positive. Conclusion The LIA reagent shows the advantage of non-specific reaction and less uncertain result. It can be considered for the routine confirmation of HIV antibody, and is continuously improved in practical application.