【摘 要】
：
10~5个/ml游离肝细胞5ml,在500r/min下离心沉淀3分钟,用吸管吸出上层培养液,然后用1640培养液重复洗涤7次。最后加重蒸水稀释至原体积。用0.5％NH_4H_2PO_4作基体改进剂,能有
【机 构】
：
湖南医科大学环境医学研究室,湖南医科大学环境医学研究室,湖南医科大学环境医学研究室 长沙 410078珠海前山新涌工业区中南方果蔬保鲜技术开发基地519075,长沙 410078,长沙 410078
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10~5个/ml游离肝细胞5ml,在500r/min下离心沉淀3分钟,用吸管吸出上层培养液,然后用1640培养液重复洗涤7次。最后加重蒸水稀释至原体积。用0.5％NH_4H_2PO_4作基体改进剂,能有效地消除干扰。用无火焰原子吸收光谱法直接测定细胞内镉。条件如下:进样10μl,氚灯扣除背景,分析线波长228.8nm,灯电流5mA,狭缝0.5nm,干燥70～
10 ~ 5 / ml free liver cells 5ml, centrifuged at 500r / min for 3 minutes, pipette the upper culture medium, and then repeated washing with 1640 medium 7 times. Finally add heavier distilled water to the original volume. With 0.5% NH_4H_2PO_4 matrix modifier, can effectively eliminate the interference. Direct Determination of Intracellular Cadmium by Flameless Atomic Absorption Spectrometry. Conditions are as follows: injection 10μl, tritium deduction of the background, analysis of line wavelength 228.8nm, lamp current 5mA, slit 0.5nm, dry 70 ~
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