论文部分内容阅读
目的:建立孕妇清火丸中黄芩苷和黄芩素的RP-HPLC含量测定方法。方法:高效液相色谱法:色谱柱为Inertsil ODS-SP-C18(4.6mm×250mm,5μm);流动相为甲醇-0.4%磷酸溶液(51:49);检测波长:277nm;流速:1.0mL.min-1;柱温:25℃。结果:黄芩苷在1.02×10-3~3.57×10-3μg范围内线性关系良好,r=0.9999;黄芩素在0.537~5.37μg范围内线性关系良好,r=0.9992;平均回收率:黄芩苷为102.78%,RSD=1.33%(n=5),黄芩素为103.27%,RSD=1.03%(n=5)。结论:本方法准确,结果可靠,重现性好,可作为孕妇清火丸质量控制的方法。
Objective: To establish a RP-HPLC method for the determination of baicalin and baicalein in pregnant women. Method: High performance liquid chromatography: Inertsil ODS-SP-C18 column (4.6mm×250mm, 5μm); mobile phase methanol-0.4% phosphoric acid solution (51:49); detection wavelength: 277nm; flow rate: 1.0mL .min-1; Column temperature: 25°C. Results: The linear relationship of baicalin was good in the range of 1.02×10-3~3.57×10-3μg, r=0.9999. The linear relationship of baicalein was good in the range of 0.537~5.37μg, r=0.9992. The average recovery rate was baicalin. 102.78%, RSD = 1.33% (n = 5), baicalein was 103.27%, RSD = 1.03% (n = 5). Conclusion: The method is accurate, reliable, and reproducible. It can be used as a method for the quality control of pregnant woman Qing Huo Pills.