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【目的】为探明不同水稻品种(丰源优272、R9810-T、华恢1号、明恢63、麻糯谷和Rathu Heenati)对白背飞虱Sogatella furcifera的抗性差异机理,利用刺吸电位技术(electronic penetration graph,EPG)记录了该虫在这6个品种三叶期稻苗上的取食行为。【方法】结合特定的EPG波形,考察了9个非韧皮部指标和22个韧皮部指标。【结果】在总记录时间8 h内,白背飞虱在品种Rathu Heenati上非刺探波np的总时间最长,其次是麻糯谷上非刺探波np的总时间,这两者间差异显著且都显著地长于其他4个品种的np总时间(P<0.05);该虫在Rathu Heenati上路径波Nc的总时间为8 523.41 s,是感虫品种明恢63 Nc总时间的2.24倍;该虫在Rathu Heenati上吸食韧皮部汁液N4-b的总时间显著地比其他品种短(P<0.05)。华恢1号和R9810-T上所有的EPG指标和感虫品种明恢63的EPG指标都没有显著的差异(P≥0.05)。白背飞虱在丰源优272上单次分泌水溶性唾液N4-a的平均时间更长,并伴随长时间的韧皮部取食。【结论】由此推测,品种Rathu Heenati可能存在不利于白背飞虱取食的忌避成分,并且在韧皮部外组织和韧皮部组织都存在抗性因子;在麻糯谷上仅存在忌避成分;然而,品种华恢1号和R9810-T可能对白背飞虱不具有明显的抗性;丰源优272可能是比明恢63更为感虫的品种。结合介体昆虫取食行为与传播持久性病毒的关系,这些结果也为利用抗白背飞虱品种控制南方水稻黑条矮缩病提供了参考。
【Objective】 To investigate the mechanism of resistance differences of Sogatella furcifera from different rice varieties (Fengyuanyou 272, R9810-T, Huahui 1, Minghui 63, Ma Nuo Valley and Rathu Heenati) The electronic penetration graph (EPG) recorded the feeding behavior of the insects on these three varieties of rice seedlings. 【Method】 Nine non-phloem indicators and 22 phloem indicators were studied with specific EPG waveforms. 【Result】 The total time of non-spiking np on the Rathu Heenati was longer than that of the white spider nymphs within 8 h of the total recording time, followed by the total time of non-spiked np on the numbness waxy valley, the difference was significant (P <0.05). The total Nc time of the worm on Rathu Heenati was 8 523.41 s, which was 2.24 times of that of the susceptible variety Minghui 63 Nc. The total time for this worm to ingest phloem juice N4-b on Rathu Heenati was significantly shorter (P <0.05) than the other breeds. There was no significant difference (P> 0.05) between the EPG index of Hua Hui 1 and R9810-T and the EPG index of the susceptible variety Minghui 63. The average time for white-backed planthopper Nilaparvata lugens to secrete water-soluble saliva N4-a on Fengyuanyou 272 was longer and accompanied by prolonged phloem feeding. 【Conclusion】 It is speculated that Rathu Heenati may have a repellent component that is detrimental to whitebacked planthopper feeding, and there are resistance factors in the tissues of phloem and phloem tissues. There are only repellent components in the hemp waxy valley. However, Varieties Huayu 1 and R9810-T may not have obvious resistance to Sogatella furcifera; Fengyuanyou 272 may be a more susceptible variety than Minghui63. Combined with the relationship between the insecticidal feeding behavior of insects and persistent virus transmission, these results also provide a reference for the control of rice black-streaked dwarf diseases in southern China with the resistance to WBPH.