应用酶标记SPA代替酶标记抗人补抗体进行抗补体免疫酶试验,检查Raji细胞,B95—8细胞和鼻咽癌病人的癌细胞均发现有EBNA阳性细胞,而Hela细胞,CNE—1细胞及66例鼻咽慢性炎症病人的鼻咽粘膜脱落细胞均未发现EBNA阳性细胞。此方法检查EBNA与曾毅等人报道的抗补体免疫酶法一样敏感和特异,而且SPA来源方便,有利于在基层开展工作。 The anti-complementing enzyme immunoassay was performed using an enzyme-labeled SPA instead of an enzyme-labeled anti-human complement antibody. EBNA-positive cells were detected in Raji cells, B95-8 cells, and NPC patients, while HeLa cells, CNE-1 cells, and No EBNA-positive cells were found in nasopharyngeal epithelial cells in 66 patients with chronic nasopharyngeal inflammation. This method checks that EBNA is as sensitive and specific as the anti-complementing enzyme immunoassay reported by Zeng Yi et al., and the source of SPA is convenient, which is conducive to work at the grassroots level.