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目的 观察延髓和脊髓背角内蛋白激酶Cγ亚单位 (PKCγ)样阳性神经元向中脑导水管周围灰质(PAG)的投射。 方法 荧光金 (FG)逆行追踪与PKCγ的免疫荧光组织化学染色相结合的双标记技术。 结果 PKCγ样阳性神经元主要分布于延髓和脊髓背角的Ⅰ、Ⅱ、Ⅲ层及脊髓的外侧脊核 ;将FG注入PAG后 ,在延髓和脊髓背角的Ⅰ、Ⅱ、Ⅲ层及脊髓的外侧脊核内可见FG标记神经元 ;部分FG标记神经元呈PKCγ样阳性 ,FG PKCγ双标神经元也主要见于延髓和脊髓背角的Ⅰ、Ⅱ、Ⅲ层及脊髓的外侧脊核。 结论 延髓和脊髓背角的PKCγ阳性神经元可能参与将伤害性刺激信息向PAG的传递。
Objective To observe the projection of protein kinase C gamma subunit (PKCγ) -like neurons in medulla oblongata and spinal dorsal horn to the periaqueductal gray (PAG). Methods Dual labeling technique of fluorescent gold (FG) retrograde tracing combined with immunofluorescence staining of PKCγ was performed. Results PKCγ-like neurons were mainly distributed in the layers Ⅰ, Ⅱ and Ⅲ of the medulla and spinal dorsal horn and the lateral spinal nucleus of the spinal cord. After FG was injected into the PAG, the neurons in the medullary and spinal dorsal horn Ⅰ, Ⅱ, Ⅲ and spinal cord FG-labeled neurons were found in the lateral spinal nucleus. Some FG-labeled neurons were positive for PKCγ-like. The double-labeled FG-PKCγ neurons were mainly found in the layers Ⅰ, Ⅱ and Ⅲ of the medulla and spinal dorsal horn and the lateral spinal nucleus of the spinal cord. Conclusion PKCγ-positive neurons in the medullary and spinal dorsal horn may be involved in the transmission of nociceptive stimuli to PAG.