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抗条锈病小麦新种质D31是以印度圆粒小麦(Triticum sphaerococcum Perc.)AS384与普通小麦品系94-3854杂交、回交选育而成的新品系。用中国小麦条锈病菌(Puccinia striiformis f.sp. stritici)流行生理小种条中32号对D31和Tai-chung29的杂交后代进行苗期及成株期抗条锈性遗传分析。结果表明,D31对生理小种条中32号表现为全生育期近免疫抗性反应,其抗性由1对隐性基因控制,暂命名为YrSph。利用BSA法对构建的F2遗传作图群体进行SSR标记分析。通过对400对微卫星引物的筛选和群体分析表明,抗性基因与Xwmc149、Xwmc246、Xgwm372和Xwmc198具有连锁关系,其中与Xwmc246标记连锁较近,遗传距离为8.8 cM。基因和标记之间的顺序为Xwmc149-YrSph-Xwmc246-Xgwm372-Xwmc198。根据作图结果,将D31所含的抗条锈病基因YrSph定位于2A短臂上。基于该基因的作图位置与系谱分析,认为该基因可能是1个新的抗条锈病基因。
The new stripe rust resistance wheat germplasm D31 is a new line derived from the cross between Triticum sphaerococcum Perc. AS384 and common wheat 94-3854. The genetic analysis of the resistance to stripe rust in seedling and adult plants of hybrid progeny of D31 and Tai-chung29 was conducted with a Chinese poppy variety Puccinia striiformis f. Sp. Stritici. The results showed that D31 showed nearly immunoreactive response to the whole growth period on Physiological Race 32, and its resistance was controlled by one recessive gene and temporarily named YrSph. The constructed F2 genetic mapping population was subjected to SSR marker analysis using BSA method. Screening and population analysis of 400 pairs of microsatellite primers showed that the resistance gene has a linkage relationship with Xwmc149, Xwmc246, Xgwm372 and Xwmc198, with the closest linkage to Xwmc246 and a genetic distance of 8.8 cM. The order between the gene and the marker is Xwmc149-YrSph-Xwmc246-Xgwm372-Xwmc198. According to the results of the mapping, the YrSph gene of D31 was located on the short arm of 2A. Based on the mapping position and pedigree analysis of this gene, it is considered that this gene may be a new stripe rust resistance gene.