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目的：寻找巨细胞病毒宫内感染产前诊断的途径。方法：应用酶联免疫吸附试验（ＥＬＩＳＡ）和聚合酶链反应（ＰＣＲ）技术，对２４例妊娠患有巨细胞病毒（ＣＭＶ）活动性感染的产妇，分娩的新生儿脐血抗ＣＭＶ－ＩｇＭ型抗体和羊水及尿液ＣＭＶ－ＤＮＡ进行检测。结果：２４例ＣＭＶ活动性感染孕妇中有１４例发生宫内感染，宫内感染率５８．３％，与对照组比较有高度显著性差异。宫内感染儿羊水ＣＭＶ－ＤＮＡ阳性率７８．６％，显著高于脐血ＣＭＶ－ＩｇＭ阳性率．孕妇尿液排毒状态与宫内感染的发生无明显关系。结论：宜将ＣＭＶ－ＩｇＭ阳性孕妇视为有宫内感染潜在危险的对象；羊水ＰＣＲ－ＣＭＶ检测可望成为产前诊断ＣＭＶ宫内感染的有效手段。
Objective: To search for prenatal diagnosis of cytomegalovirus intrauterine infection. Methods: 24 pregnant women with cytomegalovirus (CMV) active infection during pregnancy, neonatal umbilical cord blood anti-CMV-IgM type of childbirth were detected by enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction Antibodies and amniotic fluid and urine CMV-DNA were detected. Results: Intrauterine infection was found in 14 of 24 pregnant women with CMV active infection, the intrauterine infection rate was 58.3%, which was highly significant difference compared with the control group. The positive rate of CMV-DNA in intrauterine infection of amniotic fluid was 78.6%, which was significantly higher than that of CMV-IgM in cord blood. Urinary detoxification of pregnant women and intrauterine infection had no significant relationship. CONCLUSION: CMV-IgM positive pregnant women should be considered as potentially dangerous target for intrauterine infection. PCR-CMV of amniotic fluid could be an effective method to diagnose intrauterine infection of CMV prenatal.