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目的:探讨TEKT4蛋白在特发性弱精子症发生机制中的作用。方法:采用非连续密度梯度离心分离和纯化特发性弱精子症患者和正常男性精子,采用RT-PCR和Western印迹方法,从mRNA和蛋白水平检测TEKT4的表达及其差异。结果:RT-PCR结果表明,TEKT4 mRNA在特发性弱精子症患者精子中的表达水平与正常男性相比显著降低(0.59±0.13 vs 0.75±0.15,t=4.325,P<0.05);Western印迹结果与RT-PCR结果一致,TEKT4蛋白在特发性弱精子症患者精子中的表达水平与正常男性相比亦显著降低(0.48±0.14 vs 0.69±0.13,t=5.939,P<0.05)。结论:特发性弱精子症患者精子中TEKT4表达水平显著降低,可能是导致弱精子症发生的重要环节之一。
Objective: To investigate the role of TEKT4 in the pathogenesis of idiopathic asthenospermia. Methods: Non-continuous density gradient centrifugation was used to separate and purify idiopathic asthenospermia patients and normal male sperm. RT-PCR and Western blotting were used to detect the expression and difference of TEKT4 mRNA and protein. Results: RT-PCR results showed that the expression level of TEKT4 mRNA in sperm of idiopathic asthenospermia patients was significantly lower than that in normal males (0.59 ± 0.13 vs 0.75 ± 0.15, t = 4.325, P <0.05); Western blot Results The results of RT-PCR showed that the expression of TEKT4 protein in sperm of idiopathic asthenospermia patients was significantly lower than that in normal men (0.48 ± 0.14 vs 0.69 ± 0.13, t = 5.939, P <0.05). Conclusion: The expression of TEKT4 in spermatozoa of idiopathic asthenospermia patients is significantly decreased, which may be one of the important factors leading to the occurrence of asthenospermia.