谷酰胺对高体积分数氧肺损伤新生大鼠的保护作用

来源 :实用儿科临床杂志 | 被引量 : 0次 | 上传用户:yczhudong
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的探讨谷酰胺对新生大鼠高体积分数氧(高氧)肺损伤的保护作用。方法将足月2日龄新生SD大鼠24只随机均分为空气组、谷酰胺组和高氧组,每组8只。空气组新生大鼠不做处理,谷酰胺组新生大鼠实验前腹腔注射0.75 g·kg-1·d-1谷酰胺溶液,高氧组新生大鼠腹腔注射等容量9 g·L-1盐水,连续3 d;高氧组与谷酰胺组新生大鼠置于氧体积分数大于900 mL·L-1模型箱内,空气组新生大鼠置于同一室内常压空气中。分别于实验开始3 d、6 d比较各组新生大鼠体质量,于实验开始6 d无菌条件下处死新生大鼠,取肺组织,24 h后制成石蜡标本,观察病理改变及热休克蛋白70(HSP70)的表达。结果 1.高氧组6 d存活率明显低于空气组(P<0.05);谷酰胺组6 d存活率与空气组比较差异无统计学意义(P>0.05)。高氧下暴露3 d,3组体质量比较差异无统计学意义(P>0.05);高氧暴露6 d,高氧组体质量明显低于空气组(P<0.01),谷酰胺组体质量与空气组比较差异无统计学意义(P>0.05)。2.高氧组肺水肿程度最严重,其肺湿质量/干质量值明显高于空气组(P<0.05);谷酰胺组肺湿质量/干质量值与空气组比较差异无统计学意义(P>0.05)。3.高氧暴露6 d,高氧组肺泡内有出血和大量渗出的炎性细胞、大量粉染的蛋白性液体,肺泡壁严重充血水肿;谷酰胺组肺泡内极少量红细胞渗出,肺泡腔内见粉染的水肿液,肺泡壁增厚。4.高氧暴露6 d,高氧组、谷酰胺组肺组织HSP70蛋白表达量均较空气组显著增加(Pa<0.01),谷酰胺组肺组织HSP70蛋白表达量较高氧组显著增加(P<0.01)。结论预防性使用谷酰胺可明显改善高氧肺损伤新生大鼠的生存率,有利于新生大鼠的生长发育;预防性使用谷酰胺能增加高氧肺损伤新生大鼠肺组织HSP70的表达,表明谷酰胺对高氧肺损伤的保护作用与HSP70表达相关。 Objective To investigate the protective effect of glutamine on high volume fraction oxygen (hyperoxia) lung injury in neonatal rats. Methods Twenty-four newborn Sprague-Dawley rats of 2-day-old full-term were randomly divided into air group, glutamine group and hyperoxia group, with 8 rats in each group. In the air group, the newborn rats were not treated. The glutamate group was injected intraperitoneally with 0.75 g · kg -1 · d -1 glutamine solution before the experiment. The neonatal rats in the hyperoxia group were injected intraperitoneally with the same volume of 9 g · L -1 saline For 3 consecutive days. Neonatal rats in hyperoxia group and glutamine group were placed in the model tank with the volume of oxygen greater than 900 mL · L-1, and the neonatal rats in the air group were placed in the same indoor air. Neonatal rats were sacrificed on the 6th day after the start of the experiment, and the lungs were removed. After 24 hours, paraffin wax specimens were obtained and pathological changes and heat shock were observed Protein 70 (HSP70) expression. The survival rate in 6-day hyperoxia group was significantly lower than that in air group (P <0.05). There was no significant difference in 6-day survival rate between glutamine group and air group (P> 0.05). Compared with the air group (P <0.01), the body mass of the hyperoxia group was significantly lower than that of the air group (P <0.01) Compared with the air group, the difference was not statistically significant (P> 0.05). 2. The degree of pulmonary edema in hyperoxia group was the most serious, and the lung wet mass / dry mass value was significantly higher than that in air group (P <0.05). There was no significant difference in lung wet mass / dry mass value between glutamine group and air group P> 0.05). In hyperoxia group, there were bleeding inflammatory cells in the alveoli and massive exudation of inflammatory cells in the hyperoxia group. A large amount of protein-contaminated liquid was found in the alveoli, and the alveolar wall was severely hyperemic and edematous. In the glutamine group, a very small amount of erythrocyte exuded in the alveoli, See intraluminal dyed edema, alveolar wall thickening. The expression of HSP70 protein in lung tissue of hyperoxia group and glutamine group increased significantly (P <0.01) 6 days after hyperoxia exposure, while the expression of HSP70 protein in lung tissue of glutamine group increased significantly (P <0.01). Conclusion Preventive use of glutamine can significantly improve the survival rate of neonatal rats with hyperoxia-induced lung injury, is conducive to the growth and development of newborn rats; preventive use of glutamine can increase lung tissue HSP70 expression in neonatal rats with hyperoxia-induced lung injury, indicating The protective effect of glutamine on hyperoxia-induced lung injury is related to the expression of HSP70.
其他文献
病毒性脑炎(VE)是由病毒直接侵袭脑实质引起的中枢神经系统感染性疾病。小儿VE的临床表现轻重不一,其临床表现、脑损伤程度及预后与病毒类型、患儿机体免疫状态等因素有关,脑
目的分析Duchenne型假肥大肌营养不良症(DMD)患儿主要生活事件发生时间,了解DMD进展规律及主要临床特征。方法选取临床确诊436例DMD患者(经基因检测或肌肉活检证实),详细记录
小儿肌病的种类繁多,包括先天性肌病、肌营养不良、炎性肌病、代谢性肌病以及内分泌性肌病等,各种肌病的症状、体征相似。仔细询问病史,系统的体格检查以及选择针对性、特异
目的 探讨特发性枕叶癫(癎)伴睡眠期癫(癎)性电持续状态(ESES)患儿的临床表现、EEG特征、治疗反应及预后.方法 对8例特发性儿童枕叶癫(癎)伴ESES患儿的临床及EEG资料进行分析
目的探讨儿童肺炎支原体(MP)感染相关性脑梗死的发病机制、临床表现、影像学特点及预后。方法回顾性总结分析2008年6月-2011年12月本院收治的22例MP感染并脑梗死患儿的临床表
目的探讨抗精神病药物对精神分裂症患儿血清同型半胱氨酸(Hey)水平及认知功能的影响。方法将96例首发精神分裂症患儿分为非经典抗精神病药物治疗组(研究组)和经典抗精神病药物治
目的 分析抗癫癎药物(AEDs)过敏综合征的临床特点.方法 对6例服用AEDs过敏的住院患儿临床特点进行回顾性分析.结果 6例患儿服用传统AEDs 4例,其中服用苯巴比妥、卡马西平各2
目的探讨HIE患儿血清IL-1受体拮抗剂(IL-Ira)和高敏CRP(hsCRP)水平的变化及其相关性。方法选择HIE新生儿64例为HIE组。根据意识障碍、肌张力改变、原始反射异常、惊厥等临床表现
目的通过对一典型儿童脾错构瘤(SH)病例临床特征、组织病理和脾全切除术后转归的观察,提出SH并多系统免疫疾患的可能性。方法总结1例SH患儿临床资料。血常规、骨髓、Coombs检
目的探讨脓毒症新生大鼠脑组织炎性细胞因子TNF-α的表达水平及细胞外信号调节激酶(ERK)抑制剂PD98059对其表达的影响。方法行盲肠结扎穿孔术(CLP)建立脓毒症新生大鼠模型。