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目的探讨磷脂酰肌醇蛋白聚糖-3(glypican-3)在乳腺癌组织中的表达及其与乳腺癌临床病理参数的关系。方法采用RT-PCR方法检测52例乳腺癌组织及其癌旁组织中glypican-3基因的表达,计算其阳性率;并用Western blot方法检测该基因在乳腺癌组织及其癌旁组织中的蛋白表达。结果 glypican-3 mRNA在乳腺癌组织中表达率明显低于癌旁组织(P<0.05);在有淋巴结转移者中的表达率明显低于无淋巴结转移者(P<0.05);癌组织分化程度越低,glypican-3 mRNA的表达率越低(P<0.05);但其与患者的年龄、肿瘤的大小无关(P>0.05)。glypican-3蛋白在乳腺癌组织中表达亦明显低于癌旁正常组织(P<0.05),在有淋巴结转移者中的表达量明显低于无淋巴结转移者(P<0.05),癌组织分化程度越低,glypican-3蛋白的表达量越低(P<0.05)。结论 glypican-3在乳腺癌组织中的低表达可能与乳腺癌的发生发展及侵袭转移有关。
Objective To investigate the expression of glypican-3 in breast cancer and its relationship with the clinicopathological parameters of breast cancer. Methods The expression of glypican-3 gene was detected by RT-PCR in 52 cases of breast cancer and its adjacent tissues. The positive rate of glypican-3 gene was calculated. The protein expression of glypican-3 in breast cancer tissues and adjacent tissues was detected by Western blot . Results The expression of glypican-3 mRNA in breast cancer tissues was significantly lower than that in adjacent non-cancerous tissues (P <0.05). The expression rate of glypican-3 mRNA in patients with lymph node metastasis was significantly lower than that without lymph node metastasis (P <0.05) The lower the expression of glypican-3 mRNA (P <0.05), but not related to the patient’s age and tumor size (P> 0.05). The expression of glypican-3 protein in breast cancer tissues was also significantly lower than that in adjacent normal tissues (P <0.05). The expression of glypican-3 protein in patients with lymph node metastasis was significantly lower than that without lymph node metastasis (P <0.05) The lower the glypican-3 protein expression level (P <0.05). Conclusion The low expression of glypican-3 in breast cancer may be related to the occurrence, development, invasion and metastasis of breast cancer.