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目的:探讨视神经少突胶质细胞的分离方法和体外培养条件,为研究视神经损伤修复及少突胶质细胞相关课题研究奠定基础。方法:新生Wistar大鼠20只视神经取材,以DMEM/F12为基础的化学限定性培养基进行组织块培养,并用少突胶质细胞特异性标记物半乳糖脑苷脂的抗体鉴定细胞。结果:培养第3天,视神经周围出现少突胶质细胞生长,呈圆形或梭形;10d左右基本铺满盖玻片。免疫细胞化学检测该细胞为阳性细胞,纯度较高。结论:采用视神经组织并用化学限定性培养基能成功获取体外培养的少突胶质细胞,为视神经损伤修复的研究提供了先决条件。
OBJECTIVE: To investigate the method of isolation of optic nerve oligodendrocytes and the culture conditions in vitro, and lay the foundation for the study on the repair of optic nerve injury and related research on oligodendrocytes. Methods: Twenty new optic nerve of Wistar rats were taken from the optic nerve. The tissue culture was performed in DMEM / F12-based chemically defined medium. The cells were identified by the antibody of oligodendrocyte cerebroside, a specific marker of oligodendrocyte. Results: On the 3rd day of culture, oligodendrocytes grew round or fusiform around the optic nerve; Immunocytochemistry detection of the cells as positive cells, high purity. CONCLUSION: The successful obtaining of oligodendrocytes cultured in vitro by using optic nerve tissue and chemically defined media provides the precondition for the study of optic nerve injury repair.