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目的 探讨微卫星不稳定性 (MSI)与胃癌预后的关系。方法 采用聚合酶链 简单序列长度多态性 (PCR SSCP)的方法对胃癌MSI进行检测 ,明确MSI与临床病理参数 (肿瘤大小、部位、分化、Lauren′s分型、分期 )之间的关系。其次 ,应用图像细胞仪 (ICM)对胃癌组织进行细胞核DNA含量测定 ,比较MSI阳性组与MSI阴性组之间DNA含量的差异。平均DNA含量及细胞增殖指数作为观察指标。结果 5 1例胃癌MSI总阳性率为 4 3.1% (2 2 /5 1)。中 高分化腺癌MSI检出率 (6 5 .2 % )显著高于低分化腺癌 (2 5 .0 % ) (P <0 .0 1) ;肠型胃癌MSI阳性率 (5 7.7% )高于弥漫型胃癌 (2 5 .0 % ) (P <0 .0 5 )。MSI阳性组平均DNA含量、细胞增殖指数均显著低于MSI阴性组 (10 .0 16 16± 0 .96 6 77VS 16 .3314 4± 2 .972 5 3& 0 .390 6 6± 0 .0 890 1VS 0 .7395 5± 0 .10 4 33,P <0 .0 1)。结论 MSI阳性胃癌预后可能相对较好
Objective To investigate the relationship between microsatellite instability (MSI) and the prognosis of gastric cancer. Methods The gastric cancer MSI was detected by polymerase chain reaction-based simple sequence length polymorphism (PCR-SSCP), and the relationship between MSI and clinicopathological parameters (tumor size, location, differentiation, Lauren’s classification and staging) was determined. Secondly, the DNA content of gastric cancer tissue was determined by image cytometer (ICM), and the difference of DNA content between MSI-positive group and MSI-negative group was compared. The average DNA content and cell proliferation index as indicators. Results The positive rate of MSI in 51 gastric cancer cases was 4 3.1% (2 2/5 1). The detection rate of MSI was significantly higher in moderate-high differentiated adenocarcinoma (6.52%) than in poorly differentiated adenocarcinoma (25.0%) (P <0.01). The positive rate of MSI in gastric adenocarcinoma In diffuse gastric cancer (25.0%) (P <0.05). The average DNA content and cell proliferation index in MSI positive group were significantly lower than those in MSI negative group (10 .0 16 16 ± 0 .96 6 77VS 16 .3314 4 ± 2 .972 5 3 &0 .390 6 6 ± 0 .0 890 1VS 0 .7395 5 ± 0.10 4 33, P <0.01). Conclusion The prognosis of MSI positive gastric cancer may be relatively good