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目的 研究重组人生长激素 (rhGH)在体外对肿瘤细胞是否有促进增殖作用 ,为研究rhGH在体内作用及肿瘤患者如何应用rhGH作准备。方法 将不同浓度的rhGH(国产和进口两种 )加入相同的肺癌细胞培养液中 ,培育不同时间 ,用同位素计数法测量结果 ;同时作顺铂及空白对照试验。结果 未发现rhGH在体外有明显的促肿瘤细胞增殖作用。结论 rhGH在体外无促进肿瘤细胞增殖的作用 ,在体内作用如何及肿瘤患者如何安全应用rhGH还有待进一步研究。
Objective To study whether recombinant human growth hormone (rhGH) can promote the proliferation of tumor cells in vitro, in order to study the role of rhGH in vivo and how to use rhGH in tumor patients. Methods Different concentrations of rhGH (both domestic and imported) were added into the same lung cancer cell culture medium and incubated for different time. The results were measured by isotope counting method. At the same time, cisplatin and blank control were tested. Results did not find rhGH in vitro significantly promote tumor cell proliferation. Conclusion rhGH can not promote the proliferation of tumor cells in vitro, and the role of rhGH in vivo and how to safely use rhGH in tumor patients remains to be further studied.