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目的通过建立KM种小鼠实热壅滞的粪性腹膜炎(ABP)模型,观察大承气汤对实热壅滞的粪性腹膜炎小鼠内毒素及血清丙二醛(MDA)和超氧化物歧化酶(S0D)含量的影响,探索大承气汤治疗急腹症类疾病的机理。方法 KM小鼠64只,随机分为对照组、实热壅滞的粪性腹膜炎模型组、大承气汤治疗组和氨苄西林治疗组,应用专属试剂盒分别检测各组外周静脉血及回肠内容物的内毒素含量,测定血清MDA、SOD含量。结果造模各组小鼠出现口唇发绀、眼角分泌物浓稠、竖毛、少动、蜷曲蹲伏、不进食、大便极干、腹部鼓胀等症状。解剖腹腔可见腹腔内有少量浑浊液体,肠管充血,多数肠管膨胀,部分肠管痉挛。大承气汤组与模型组相比,外周血及回肠内容物中的内毒素含量、血清MDA含量均明显降低(P<0.05),SOD含量显著升高(P<0.01),但氨苄西林组与模型组相比上述指标差异不明显(P>0.05)。结论大承气汤能抑制实热壅滞的粪性腹膜炎小鼠内毒素位移和氧化-抗氧化失衡。
Objective To establish a model of fecal peritonitis (ABP) in mice with KM mice and to observe the effect of Dachengqi Decoction on the endotoxin and serum malondialdehyde (MDA) and superoxide in mice with febrile peritonitis. The influence of dismutase (S0D) content on the mechanism of Dachengqi Decoction in the treatment of acute abdomen diseases. Methods A total of 64 KM mice were randomly divided into control group, fecal peritonitis model group, Dachengqi Decoction group and ampicillin treatment group. The contents of peripheral venous blood and ileum in each group were detected using special kits. The endotoxin content of the substance was measured in serum MDA and SOD. RESULTS: The mice in each group were found to have cyanotic cyanosis, thick secretions on the corners of the eyes, erect hairs, little movement, crooked cheeks, no eating, very dry stools, and abdominal bulging. Anatomical abdominal cavity visible a small amount of turbid fluid in the abdominal cavity, intestinal congestion, most of the intestine expansion, part of the intestinal fistula. Compared with the model group, the contents of endotoxin and serum MDA in the peripheral blood and ileum contents of Dachengqi Decoction group were significantly lower (P<0.05), SOD content was significantly increased (P<0.01), but the ampicillin group Compared with the model group, the above indicators showed no significant difference (P>0.05). Conclusion Dachengqi Decoction can inhibit the endotoxin translocation and oxidative-antioxidative imbalance in mice with febrile peritonitis with hyperthermia.