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目的 探讨CacyBP在胃癌多药耐药机制中的作用。方法 构建CacyBP正义核酸真核表达载体pcDNA3.1/hCacyBP +,以脂质体将其导入胃癌药敏细胞SGC790 1。以RT PCR检测hCacyBPmRNA水平的变化 ;以MTT检测SGC790 1及转染细胞对ADR的药物敏感性 ;以流式细胞仪 (FCM)检测SGC790 1及转染细胞内ADR的蓄积浓度及细胞周期。结果 转染pcDNA3.1/hCacyBP +的SGC790 1,其hCacyBPmRNA表达水平显著高于空载体转染及未转染之SGC790 1,且细胞生存率亦有所增高。未转染之SGC790 1及分别转染空载体或pcDNA3.1/hCacyBP +之SGC790 1细胞 ,平均ADR蓄积浓度分别为 5 .6 4 ,5 .4 9和 5 .17。与未转染之SGC790 1相比 ,转染pcDNA3.1/hCacyBP +和空载体的SGC790 1细胞G1期减少 ,G2 期及S期增高。结论 CacyBP在胃癌MDR机制中可能有一定作用
Objective To investigate the role of CacyBP in multidrug resistance of gastric cancer. Methods CacyBP eukaryotic expression vector pcDNA3.1 / hCacyBP + was constructed and introduced into gastric cancer cell line SGC7901 by liposome. The changes of hCacyBP mRNA level were detected by RT PCR. The drug sensitivity of SGC790 1 and transfected cells to ADR was detected by MTT assay. The accumulation concentration and cell cycle of SGC790 1 and transfected cells were detected by flow cytometry (FCM). Results The expression level of hCacyBP mRNA in SGC790 1 transfected with pcDNA3.1 / hCacyBP + was significantly higher than that in SGC7901 transfected with empty vector and non-transfected cells, and the cell survival rate also increased. The average ADR accumulation concentration of untransfected SGC7901 cells and SGC7901 cells transfected with empty vector or pcDNA3.1 / hCacyBP + were respectively 5.44,5.49 and 5.17. SGC7901 cells transfected with pcDNA3.1 / hCacyBP + and empty vector showed a decrease of G1 phase and an increase of G2 phase and S phase as compared with non-transfected SGC7901 cells. Conclusion CacyBP may play a role in the mechanism of gastric cancer MDR