本文报告有关1981年冬季杭州地区婴幼儿秋季腹泻的病因调查研究结果:(一)33例患儿粪便标本经纯化处理,直接电镜观察,29例找到轮状病毒颗粒,检出率为87.87%;有2例粪便电镜阴性,而血清补结反应呈阳性,总检出率为93.93%(31/33);同期采集同年龄组正常儿20人粪便标本,有2例找到中病毒;(二)上述33例患儿粪便,其中31份一分为三,二份保存-10℃,一份保存4℃冰箱。4℃保存者,电镜观察,有25份找到轮状病毒颗粒,说明一般冰箱保存的粪便标本,在45天以内,仍可作本病毒的病原学研究。 The paper reports about the etiology of autumn diarrhea in infants and young children in Hangzhou area in 1981: (1) 33 cases of stool samples were purified and observed by direct electron microscopy. The detection rate of rotavirus particles was 87.87% in 29 cases. There were 2 cases of negative stool electron microscopy, but the serum complement reaction was positive, with a total detection rate of 93.93% (31/33). In the same period, 20 stool samples of normal children of the same age group were collected, The above 33 cases of feces in children, of which 31 were divided into three, two preserved -10 ℃, a preserved 4 ℃ refrigerator. 4 ℃ depositors, electron microscopy, there are 25 rotavirus particles found, stool samples stored in the general refrigerator, within 45 days, still can be used for the etiological research of the virus.