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[目的]探讨用放射性碘标记的框架区mRNA(framework region,FR mRNA)反义寡核苷酸(antisense oligonucleotide,ASONs)作为淋巴瘤的反义显像剂的可能性。[方法]合成5′含酪胺的硫磷修饰的18聚体寡核苷酸,并通过氯胺-T法用放射性碘标记。用脂质体包裹125I-FR-ASONs转导Namalwa(V1家族B细胞淋巴瘤)和HL-60(人髓性白血病)细胞,并比较转导效率。荷瘤BALB/C鼠瘤内注入脂质体包裹的131I-FR-ASONs,分别于注射后0、1、2、24h进行SPECT显像。免疫组化分析肿瘤组织Bcl-2表达情况。[结果]5′含酪胺的ASONs能用放射性碘稳定标记,在Namalwa细胞中的转导效率达26.8%±1.54%。反义探针瘤内注入荷Namalwa细胞裸鼠中,示踪剂特异性浓聚在肿瘤组织。免疫组化分析表明反义组肿瘤组织Bcl-2染色较弱。[结论]放射性碘标记的FRASONs基因可特异性识别V1家族B细胞淋巴瘤,有望用于淋巴瘤反义显像和反义治疗的研究。
[Objective] To explore the possibility of using antisense oligonucleotide (FRNA) framework region (FR mRNA) antisense oligonucleotide (ASONs) as antisense imaging agent for lymphoma. [Method] The 5 ’tyrosine-containing 18-mer oligonucleotide modified with tyramine was synthesized and labeled with radioactive iodine by chloramine-T method. 125I-FR-ASONs were transplanted with liposomes to transduce Namalwa (V1 family B-cell lymphoma) and HL-60 (human myeloid leukemia) cells and compare transduction efficiency. The tumor-bearing BALB / c mice were intratumorally injected with liposome-encapsulated 131I-FR-ASONs, SPECT imaging was performed at 0, 1, 2, 24 h after injection. Immunohistochemical analysis of tumor tissue Bcl-2 expression. [Result] The 5 ’tyramine-containing ASONs could be stably labeled with radioactive iodine and the transduction efficiency in Namalwa cells was 26.8% ± 1.54%. Antisense probes were injected intratumorally into nude mice bearing Namalwa cells, and the tracer specifically accumulated in the tumor tissue. Immunohistochemical analysis showed that antisense group of tumor tissue Bcl-2 staining is weak. [Conclusion] The radioactive iodine labeled FRASONs can specifically recognize V1 family B cell lymphoma, which is expected to be useful for antisense imaging and antisense therapy of lymphoma.