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目的探讨利用脑胶质瘤分离培养胶质瘤干细胞的方法,并建立脑胶质瘤浸润动物模型。方法采用神经干细胞培养方法对12例手术切除的脑胶质瘤新鲜肿瘤组织进行培养,对形成细胞球的干细胞进行增殖、自我更新及分化试验,用免疫细胞化学法检测干细胞标志物,并用转染增强型绿色荧光蛋白的干细胞进行体内成瘤试验。结果 12例胶质瘤标本中共有9例成功培养出肿瘤神经细胞球,这些神经细胞球具有增殖、自我更新及分化能力,能表达特异性的分子标记物,并且可在免疫缺陷小鼠脑内形成与人类胶质瘤相似的、具有浸润性特征的肿瘤。结论采用神经干细胞无血清培养方法可以成功培养出胶质瘤干细胞,并且在体内建立了一种新的胶质瘤浸润模型。
Objective To investigate the method of using glioma to isolate and culture glioma stem cells and to establish glioma-infiltrating animal model. Methods The neural stem cell culture method was used to culture fresh tumor tissue of 12 patients with surgically resected glioma. The proliferation, self-renewal and differentiation of stem cells were detected by immunocytochemistry. The stem cell markers were detected by transfection Enhanced green fluorescent protein-derived stem cells in vivo tumorigenicity test. Results A total of 9 glioma samples were obtained from the 12 glioma samples. The neurospheres had proliferative, self-renewal and differentiation capabilities, and could express specific molecular markers, and could be expressed in the brain of immunodeficient mice Form tumors that are similar to human gliomas and have invasive features. Conclusion Glioma stem cells can be successfully cultivated by serum-free culture of neural stem cells, and a new glioma infiltration model has been established in vivo.