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目的 :研究三氧化二砷 (As2 O3 )在体外是否具有抗肝癌作用 ,并对其作用机制进行初步探讨。方法 :用不同浓度的As2 O3 处理Bel 740 2人肝癌细胞株及L 0 2正常人肝细胞株 ,应用四甲基偶氮唑盐比色法 (MTT法 )观察As2 O3 对细胞生长的影响 ,并用电镜、流式细胞仪、DNA电泳等检测方法研究其作用机制。结果 :MTT法发现As2 O3 0 .5～ 2 .0mg·L-1的浓度均可显著抑制Bel 740 2人肝癌细胞株的生长 ,而对L 0 2正常人肝细胞株的抑制作用较弱。经As2 O3 作用后 ,透射电镜和扫描电镜观察均显示Bel 740 2细胞发生了显著的凋亡形态改变 ;提取细胞DNA行琼脂糖凝胶电泳可见典型的梯形条带 ;流式细胞仪分析可见有亚G1峰出现 ,凋亡率呈时间和剂量双重依赖性特点。结论 :As2 O3 在体外可显著抑制肝癌细胞株生长 ,其作用机制主要是诱导肿瘤细胞凋亡
Objective: To study whether As2O3 has anti-hepatocarcinoma effect in vitro and to discuss its mechanism of action. METHODS: Bel 740 2 human hepatoma cell lines and L 0 2 normal human hepatocyte cell lines were treated with different concentrations of As 2 O 3 , and MTT assay was used to observe the effect of As 2 O 3 on cell growth. Electron microscopy, flow cytometry, DNA electrophoresis and other detection methods to study its mechanism of action. RESULTS: MTT assay showed that As2O3 concentrations of 0.5 to 2.0 mg·L-1 could significantly inhibit the growth of Bel 740 2 human hepatocellular carcinoma cell lines, but the inhibition of L 0 2 normal human hepatocyte cell lines was weak. After As2O3 treatment, transmission electron microscopy and scanning electron microscopy showed that there was a significant apoptotic morphological change in Bel 740 2 cells; extraction of cellular DNA showed a typical trapezoidal band on agarose gel electrophoresis; flow cytometry analysis showed that The appearance of sub-G1 peaks showed a time-and dose-dependent duality of apoptosis rate. Conclusion: As2O3 can significantly inhibit the growth of hepatocellular carcinoma cell lines in vitro. Its mechanism of action is mainly to induce apoptosis of tumor cells.