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目的:观察桂枝麻黄各半汤、银翘散、新加香薷饮对甲1、甲3型流感小鼠白细胞介素2(IL-2)、T淋巴细胞亚群的影响。方法:选取SPF级NIH小鼠,毒种流水冲浸融化,小鼠经乙醚吸入麻醉后,病毒滴鼻每只约0.05mL。在病毒攻击前1d开始给药,以3种解表方中药组分别按最适合有效剂量(0.4mL·d-1)ig给药5d。至造模后第4d,球后取血,测IL-2和T淋巴细胞亚群CD3+,CD4+,CD8+。结果:感染甲1、甲3型流感病毒后,外周血IL-2的浓度明显降低,与正常组比较,差异有统计学意义(P<0.05)。银翘散对甲3型流感感染小鼠IL-2升高无统计学意义,病毒唑组及3种解表方法均能提高病毒感染小鼠外周血IL-2水平,且在甲1型实验中,中药治疗各组均高于正常对照组水平,差异有统计学意义(P<0.05)。感染甲1型流感病毒后,小鼠外周血中T淋巴细胞CD3+,CD4+降低,与正常对照组比较,差异有统计学意义(P<0.05),尤其CD4+降低明显,致CD4+/CD8+比例下降,与正常对照组比较,差异有统计学意义(P<0.05),提示细胞免疫功能下降失调。而3种解表方法中药均能提高CD3+,CD4+总数,与模型对照组比较差异有显著性意义(P<0.05),3治疗组CD8+也较正常组高(P<0.05),但3组的CD4+/CD8+比值较模型对照组高(P<0.05),已较接近正常水平。结论:3种解表方(桂枝麻黄各半汤、银翘散和新加香薷饮)均能不同程度地提高机体细胞免疫功能,达到抗流感病毒的效果。
Objective: To observe the effects of Guizhi Mahuang Decoction, Yinqiao Powder and Xinjiaxiangyin on interleukin-2 (IL-2) and T-lymphocyte subsets of mice with influenza A and A. METHODS: SPF-grade NIH mice were selected. The virus was leached and thawed. After the mice were anesthetized with ether, about 0.05 mL of virus was injected into the nose. Drug administration was started 1 d before virus challenge, and three kinds of Chinese herbal medicines were administered at the most suitable effective dose (0.4 mL·d-1) for 5 d. At the 4th day after modeling, blood was taken after the balloon and IL-2 and T lymphocyte subsets CD3+, CD4+, and CD8+ were measured. RESULTS: After the infection of A1 and A3 influenza virus, the concentration of IL-2 in the peripheral blood was significantly reduced, and there was a significant difference compared with the normal group (P<0.05). Yinqiao Powder had no significant increase in IL-2 in mice infected with influenza A3. The Ribavirin group and three methods of resolving the epitopes all increased IL-2 levels in peripheral blood of mice infected with the virus. The Chinese medicine treatment group was higher than the normal control group, the difference was statistically significant (P <0.05). After infection with influenza A virus, CD3+ and CD4+ of T lymphocytes in peripheral blood of mice decreased, compared with the normal control group, the difference was statistically significant (P<0.05), especially CD4+ decreased significantly, and the proportion of CD4+/CD8+ decreased. Compared with the normal control group, the difference was statistically significant (P<0.05), suggesting a declining cellular immune function. The three methods of decolonization could increase the total number of CD3+ and CD4+, which was significantly different from the model control group (P<0.05). CD8+ was also higher in the treatment group than in the normal group (P<0.05), but in the three groups The ratio of CD4+/CD8+ was higher than that of the model control group (P<0.05), and it was already close to normal. Conclusion: Three kinds of Jie-Ping recipes (Guizhi Mahuang Decoction, Yinqiao Powder and Xinjiaxiangxie Decoction) can improve the cellular immune function in different degrees to achieve the effect of anti-influenza virus.