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目的:探讨新疆地区汉族和维吾尔族慢性浅表性胃炎、慢性萎缩性胃炎、胃溃疡、胃癌患者感染幽门螺杆菌(H.pylori)细胞毒素相关基因(cagA)的分布特征及与胃相关性疾病的关系。方法:从92例胃相关性疾病的患者(汉族46例、维吾尔族46例)胃镜活检标本中分离培养H.pylori,抽提各菌株的总DNA,采用特异性引物进行PCR扩增检测H.pylori的cagA基因,并分析其分布特征及与胃相关性疾病的关系。结果:92株H.pylori菌株中cagA基因的检出率为84.8%,在所研究的4种疾病中汉族和维吾尔族患者感染的H.pylori cagA基因检出率差异无统计学意义(P>0.05);汉族和维吾尔族患者4种特定疾病的H.pylori cagA基因检出率差异无统计学意义(P>0.05)。结论:新疆地区汉族和维吾尔族间H.pylori cagA基因阳性率未见明显差异,特定胃相关性疾病间H.pylori cagA基因阳性率也未见明显差异。
Objective: To investigate the distribution of cagA gene of Helicobacter pylori (H. pylori) infected by chronic superficial gastritis, chronic atrophic gastritis, gastric ulcer and gastric cancer in Han and Uygur nationalities in Xinjiang and its relationship with gastric-related diseases Relationship. Methods: H.pylori was isolated and cultured from 92 gastroscopic biopsies of 46 patients with gastric-related diseases (46 in Han and 46 in Uygur). The total DNA of each strain was extracted and the specific primers were used for PCR amplification. pylori cagA gene, and analyze its distribution characteristics and the relationship between gastric-related diseases. Results: The detection rate of cagA gene in 92 H.pylori strains was 84.8%. There was no significant difference in the detection rate of H.pylori cagA gene among Han and Uygur patients among the 4 diseases studied (P> 0.05). There was no significant difference in the detection rate of H.pylori cagA gene among 4 specific diseases in Han and Uigur patients (P> 0.05). Conclusion: There was no significant difference in the positive rate of H.pylori cagA gene between Han and Uighur ethnic groups in Xinjiang. There was no significant difference in the positive rate of H.pylori cagA gene between specific gastric-related diseases.