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目的:探讨miR-92a与肝细胞癌的相关性,及其表达改变对肝细胞癌HepG2细胞凋亡的影响。方法:实时定量PCR检测肝细胞癌和癌旁正常组织中miR-92a的表达;将miR-92a的抑制物miR-92a inhibitor转染了肝细胞癌HepG2细胞,然后检测了转染后细胞的凋亡。结果:与癌旁正常组织相比,肝细胞癌组织中miR-92a的表达显著上调;转染后miR-92a inhibitor组的凋亡率显著增加。结论:miR-92a与肝细胞癌的发生相关,抑制其表达能够促进肝细胞癌细胞凋亡,在肝细胞癌中发挥癌基因的作用。
Objective: To investigate the relationship between miR-92a and hepatocellular carcinoma (HCC) and its effect on the apoptosis of HepG2 hepatocellular carcinoma. Methods: Real-time quantitative PCR was used to detect the expression of miR-92a in hepatocellular carcinoma and adjacent normal tissues. The miR-92a inhibitor miR-92a inhibitor was transfected into HepG2 hepatocellular carcinoma cells, Death. Results: The expression of miR-92a in hepatocellular carcinoma was significantly up-regulated compared with the adjacent normal tissues. The apoptosis rate of miR-92a inhibitor group was significantly increased after transfection. Conclusion: miR-92a is associated with the occurrence of hepatocellular carcinoma, which inhibits the expression of miR-92a and promotes the apoptosis of hepatocellular carcinoma cells and plays an oncogene role in hepatocellular carcinoma.