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目的:探讨吐温80作为Am es试验中样品溶剂的可行性。方法:分别以二甲基亚砜和含25%吐温80的水溶液为溶剂,进行蜂胶类、鱼油类、辅酶Q10类产品的溶解度试验和Am es试验。结果:蜂胶类、鱼油类、辅酶Q10类产品在二甲基亚砜中的溶解度分别为在含25%吐温80的水溶液中溶解度的23.30%、37.26%和26.73%。两种溶剂对TA97、TA98、TA100和TA102菌株均无毒性;同一样品5个剂量组在不同溶剂条件下的实验结果均无显著性差别;三种样品的Am es试验结果均为阴性。结论:吐温80是一种较为理想的Am es试验样品溶剂。
Objective: To investigate the feasibility of Tween 80 as a sample solvent in Am es test. Methods: Solubility test and am es test of propolis, fish oil and coenzyme Q10 were carried out with dimethylsulfoxide and 25% Tween 80 as solvent respectively. Results: The solubility of propolis, fish oil and coenzyme Q10 in dimethyl sulfoxide were 23.30%, 37.26% and 26.73%, respectively, in the aqueous solution containing 25% Tween 80. The two solvents were non-toxic to TA97, TA98, TA100 and TA102 strains. There was no significant difference in the results of five solvents in the same sample with different solvents. The Am es test results of all three samples were negative. Conclusion: Tween 80 is an ideal Am es test sample solvent.