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目的观察南非特有植物线叶金雀花对小鼠脑缺血再灌注损伤的保护作用。方法将小鼠随机分为对照组,脑缺血再灌注损伤组,线叶金雀花低剂量组(2.8 g/kg)、中剂量组(5.6 g/kg)、高剂量组(11.2 g/kg)。造模前预防性灌胃给药7 d,每日1次,对照组及脑缺血再灌注组给予等体积的蒸馏水。给药1周后,采用小鼠大脑中动脉栓塞法(MCAO)制备模型,模型制备成功后继续按上述方式灌胃给药7 d。通过2,3,5一氯化三苯基四氮唑(TTC)染色,观察线叶金雀花对脑损伤梗死体积的影响;通过神经功能评分实验、平衡木实验、Morris水迷宫实验、转角实验等,观察线叶金雀花对运动、认知、感觉等功能的影响;通过检测脑组织中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量,观察药物对脑损伤以后氧化还原状态的影响;通过检测脑组织中白介素1β(IL-1β)、肿瘤坏死因子(TNF-α)、细胞间粘附分子-1(ICAM-1)含量,观察药物对脑损伤以后炎症状态的影响。结果与脑缺血再灌注损伤组相比,线叶金雀花组小鼠脑梗死体积显著减少(P<0.05),平衡木得分显著降低(P<0.01),转角向非损伤侧次数显著减少(P<0.01),脑组织TNF-α含量、IL-1β含量与ICAM-1含量显著降低(P<0.01或P<0.05)。结论线叶金雀花对小鼠脑缺血再灌注损伤有一定保护作用,其作用可能与减少脑组织炎症因子有一定关系。
Objective To observe the protective effect of Grifola frondosa on cerebral ischemia-reperfusion injury in mice. Methods Mice were randomly divided into two groups: control group, cerebral ischemia-reperfusion injury group, low dose group of Vitex negundo (2.8 g / kg), middle dose group (5.6 g / kg). Pre-model prophylactic gavage administered 7 d, 1 day, the control group and cerebral ischemia-reperfusion group given an equal volume of distilled water. One week after the administration, the model was established by middle cerebral artery occlusion (MCAO) in mice. After the model was successfully established, the rats were administered with gavage for 7 days. The effects of Cymbidium fortunei on brain infarction volume were observed by TTC staining of 2,3,5-trichloroethane (TTC). Neurological function scores, balance beam, Morris water maze test, angle test The effects of leaf vetch on exercise, cognition and sensory function were observed. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), c The contents of MDA and MDA in the brain were measured. The effects of the drugs on the redox state after brain injury were observed. The levels of IL-1β, TNF-α, ICAM- -1) content, observe the impact of drugs on inflammatory state after brain injury. Results Compared with cerebral ischemia-reperfusion injury group, the volume of cerebral infarction in the leaves of Cyatheye group decreased significantly (P <0.05), the balance beam score decreased significantly (P <0.01), and the number of non-injured side decreased significantly P <0.01). The levels of TNF-α, IL-1β and ICAM-1 in brain tissues were significantly decreased (P <0.01 or P <0.05). Conclusion Viburnum bungeana has a protective effect on cerebral ischemia-reperfusion injury in mice and its effect may be related to the reduction of inflammatory factors in brain tissue.