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目的研究睾酮对地塞米松所致大鼠骨骼肌萎缩的治疗作用以及与胰岛素样生长因子1(IGF-1)表达的关系。方法40只雌性SD大鼠随机均分为对照组、地塞米松组、睾酮组、睾酮+地塞米松组,每日检测大鼠体重,末次给药24h后处死动物,冻存血浆标本,分离腓肠肌、称重并冻存。采用定量PCR检测腓肠肌中IGF-1 mRNA的表达,ELISA法检测血浆中IGF-1蛋白水平。结果睾酮可减轻地塞米松所致的大鼠体重与骨骼肌重量降低(P<0.01),拮抗地塞米松引起的骨骼肌中IGF-1 mRNA水平下降(P<0.01),但对血浆中IGF-1蛋白水平无明显影响。结论睾酮可减轻地塞米松所致的大鼠骨骼肌萎缩,对骨骼肌中IGF-1表达的调控可能是雄激素治疗作用的机制之一。
Objective To investigate the therapeutic effect of testosterone on skeletal muscle atrophy induced by dexamethasone and its relationship with the expression of insulin-like growth factor 1 (IGF-1) in rats. Methods 40 female SD rats were randomly divided into control group, dexamethasone group, testosterone group and testosterone + dexamethasone group. The body weight of rats was measured daily. After the last administration, the animals were killed and the plasma samples were frozen and separated Gastrocnemius, weighed and stored frozen. Quantitative PCR was used to detect the expression of IGF-1 mRNA in gastrocnemius muscle and IGF-1 protein in plasma by ELISA. Results Testosterone could reduce the body weight and skeletal muscle weight of rats induced by dexamethasone (P <0.01), decrease the level of IGF-1 mRNA in skeletal muscle induced by dexamethasone (P <0.01) -1 protein level had no significant effect. Conclusion Testosterone can reduce skeletal muscle atrophy induced by dexamethasone in rats and regulation of IGF-1 expression in skeletal muscle may be one of the mechanisms of androgen therapy.