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为研究5-羟色胺(5-HT)增多是否通过影响肠黏膜屏障引起断奶仔鼠的腹泻,检测断奶腹泻仔鼠小肠绒毛高度(VH)、隐窝深度(CD)变化。采用21d刚断奶ICR仔鼠,分为6组,第1组为正常对照组,第2组为应激腹泻组,番泻叶(0.4kg/L)按体重15mL/kg灌胃同时后肢束缚应激处理,第3组为氢溴酸西肽普兰(CH)对照组,用CH进行腹腔注射处理,第4组CH+腹泻组,CH药物处理4h后进行应激腹泻处理;第5组对氯苯丙氨酸(PCPA)对照组,PCPA腹腔注射;第6组PCPA+应激腹泻组,PCPA药物处理4h后进行应激腹泻处理。连续处理5日后处死小鼠,取十二指肠、空肠、回肠,4%多聚甲醛固定、石蜡包埋,制作石蜡切片,HE染色,测量肠绒毛高度、隐窝深度。由HE染色结果发现,应激腹泻组、CH+应激腹泻组和PCPA+应激腹泻组与正常对照组相比,肠绒毛顶端出现充血,绒毛变短,裸露固有层等病理现象。与正常对照组相比,应激性腹泻组小鼠肠绒毛高度降低:十二指肠,空肠,回肠分别降低14.80%(P<0.01),6.89%(P<0.01)和19.31%(P<0.01),隐窝深度分别升高14.09%(P<0.01)、4.85%(P<0.05)、11.53%(P<0.01),故VH和CD的比值(V/C)降低:CH处理组与应激腹泻组小鼠变化趋势相同;而PCPA处理组则无明显变化。与应激腹泻小鼠相比,CH+应激腹泻组小鼠肠绒毛高度降低,尤其空肠显著降低11.18%(P<0.01),隐窝深度升高,尤其十二指肠显著升高4.86%(P<0.05),故V/C值也降低,而PCPA+应激腹泻组小鼠绒毛高度升高,分别为4.83%(P<0.05),4.15%(P<0.01),10.60%(P<0.01),隐窝深度降低,分别为27.46%(P<0.01)、19.21%(P<0.01)、16.74%(P<0.01),故V/C值升高。断奶腹泻仔鼠小肠黏膜机械屏障遭到破坏,而肠道内5-HT含量的升高也会使断奶仔鼠出现腹泻现象,同时小肠绒毛高度降低,隐窝深度增加。因此5-HT升高可能通过损伤断奶仔鼠小肠的绒毛高度和隐窝深度,从而引发腹泻。
To investigate whether 5-HT increased the intestinal villus height (VH) and crypt depth (CD) in weanling offspring by diarrhea caused by affecting the intestinal mucosal barrier. The 21-day-old weaning ICR offspring were randomly divided into 6 groups: group 1 was normal control group, group 2 was stress diarrhea group, senna (0.4kg / L) Group 3 was treated with xipeptidine hydrobromide (CH) control group, intraperitoneally injected with CH, group 4 with CH + diarrhea, and treated with CH for 4 hours. The rats in group 5 were treated with chlorobenzene Alanine (PCPA) control group, PCPA intraperitoneal injection; group 6 PCPA + stress diarrhea group, PCPA drug treatment 4h after stress diarrhea. The mice were sacrificed 5 days after the continuous treatment. The duodenum, jejunum and ileum were fixed in 4% paraformaldehyde and embedded in paraffin. Paraffin sections were made and HE staining was performed to measure the villus height and crypt depth. The result of HE staining showed that compared with the normal control group, the diarrhea group, the CH + stress diarrhea group and the PCPA + stress diarrhea group showed hyperplasia, villus shortening and exposed lamina propria pathological phenomena. Compared with the normal control group, the intestinal villi in stress diarrhea group decreased significantly: duodenum, jejunum and ileum decreased 14.80% (P <0.01), 6.89% (P <0.01) and 19.31% (P < 0.01). The crypt depth increased by 14.09% (P <0.01), 4.85% (P <0.05) and 11.53% (P <0.01), respectively. The trend of the mice in the stress diarrhea group was the same, but there was no significant change in the PCPA group. Compared with the stress-induced diarrhea mice, the intestinal villus in CH + stress diarrhea group was significantly decreased, especially in jejunum (11.18%) (P <0.01), crypt depth increased, especially duodenum increased 4.86% (P <0.05), and the V / C value also decreased. The villus height in PCPA + diarrhea group was 4.83% (P <0.05), 4.15% (P <0.01) and 10.60% ), And the depth of crypts decreased 27.46% (P <0.01), 19.21% (P <0.01), and 16.74% (P <0.01), respectively. Weanling diarrhea in mice intestinal mucosal mechanical barrier was damaged, and intestinal 5-HT levels will also make diarrhea in weaning offspring phenomenon, while reducing the height of the villi, crypt depth increased. Thus, 5-HT elevation may cause diarrhea by damaging the villus height and crypt depth in the small intestine of weanling offspring.