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目的了解30株多药耐药鲍氏不动杆菌(MDRAB)的菌株亲缘性。方法对该组MDRAB完成了2种与耐药相关的管家基因和49种水平转移获得与β-内酰胺类、氨基糖苷类、喹诺酮类耐药相关基因,以及13种接合性质粒、转座子、插入序列、整合子等可移动遗传元件遗传标记检测,并对检测结果作了样本聚类分析。结果 30株MDRAB多态性明显,多数菌株已发生演化;只有A群与B群为克隆传播,水平转移基因检测阳性的模式可分15种。结论与耐药相关的管家基因和水平转移获得的耐药基因均为显性遗传,与我们研究耐药菌所观察的表型相对应,为追溯耐药菌传播途径提供了方便。
Objective To understand the phylogenetic relationships among 30 multidrug-resistant Acinetobacter baumannii (MDRAB) strains. Methods Two MDRAB-resistant housekeeping genes and 49 horizontal transplants were obtained in this group, and the genes related to resistance to β-lactams, aminoglycosides and quinolones were obtained, and 13 conjugative plasmids, transposons , Insert sequences, integrons and other portable genetic elements genetic markers detection, and the test results were clustered analysis. Results The 30 MDRAB polymorphisms were obvious and most of the strains had been evolved. Only group A and group B were cloned and the patterns of positive gene of horizontal metastasis were divided into 15 species. Conclusion Both the housekeeping genes associated with drug resistance and the drug resistance genes obtained by horizontal transfer are dominantly inherited, which correspond to the phenotypes observed by drug-resistant bacteria and provide a convenient way to trace the route of transmission of drug-resistant bacteria.