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目的:研究辅助生殖技术中常用物品对精子活动率的影响。方法:采用人精子存活试验检测两种品牌的培养皿、两种品牌的注射器和用IVF专用培养液或生理盐水冲洗前后的取卵针、胚胎移植管及医用橡胶手套,比较各组两种品牌产品对人精子活率的影响。结果:丹麦Nunclon公司的四孔组织培养皿和美国Falcon公司的单孔井型培养皿之间精子活率比较,统计学分析差异有显著性(52.68±16.21vs45.36±15.25,P<0.01)。美国BD、国产洁瑞两种品牌的注射器之间精子活率比较,统计学分析差异无显著性(P>0.05)。取卵针、胚胎移植管、医用手套冲洗前后精子活率比较,统计学分析差异均无显著性(P>0.05)。结论:相同用途的辅助生殖用品不同品牌人精子活率可能有不同或相同的影响,所取品牌取卵针、胚胎移植管、医用手套冲洗与否对生殖细胞和胚胎影响无差异,可根据质控结果选择适合本实验室的用品以及适宜的使用方法。
Objective: To study the effect of commonly used items in assisted reproductive technology on sperm motility. Methods: The human sperm survival test was used to detect two brands of Petri dishes, two brands of syringes and ovum pins, embryo transfer tubes and medical rubber gloves before and after washing with IVF special culture solution or saline. Effect of product on human sperm viability. Results: There was a significant difference in sperm viability between the four-well tissue culture dish of Nunclon and the single-well well culture dish of American Falcon Company (52.68 ± 16.21 vs 45.36 ± 15.25, P <0.01) . There was no significant difference in sperm viability between American BD and domestic Jerry brand syringes (P> 0.05). Oviposition needles, embryo transfer tubes and medical gloves before and after rinsing showed no significant difference in statistical analysis (P> 0.05). CONCLUSIONS: The sperm motility of different brands of assisted reproductive articles for the same purpose may have different or same effects on the sperm motility. The fetching of needles, embryo transfer tubes and medical gloves of the brands with the same purpose have no difference on germ cells and embryos, Control the results of choice for the laboratory supplies and the appropriate use of methods.